TY - JOUR
T1 - Generation of stem cell-based bioartificial anterior cruciate ligament (ACL) grafts for effective ACL rupture repair
AU - Kouroupis, Dimitrios
AU - Kyrkou, Athena
AU - Triantafyllidi, Eleni
AU - Katsimpoulas, Michalis
AU - Chapelakis, George
AU - Goussia, Anna
AU - Georgoulis, Anastasios
AU - Murphy, Carol
AU - Fotsis, Theodore
PY - 2016/4/22
Y1 - 2016/4/22
N2 - In the present study, we combined stem cell technology with a non-absorbable biomaterial for the reconstruction of the ruptured ACL. Towards this purpose, multipotential stromal cells derived either from subcutaneous human adipose tissue (hAT-MSCs) or from induced pluripotent stem cells (iPSCs) generated from human foreskin fibroblasts (hiPSC-MSCs) were cultured on the biomaterial for 21 days in vitro to generate a 3D bioartifical ACL graft. Stem cell differentiation towards bone and ligament at the ends and central part of the biomaterial was selectively induced using either BMP-2/FGF-2 or TGF-β/FGF-2 combinations, respectively. The bioartificial ACL graft was subsequently implanted in a swine ACL rupture model in place of the surgically removed normal ACL. Four months post-implantation, the tissue engineered ACL graft generated an ACL-like tissue exhibiting morphological and biochemical characteristics resembling those of normal ACL.
AB - In the present study, we combined stem cell technology with a non-absorbable biomaterial for the reconstruction of the ruptured ACL. Towards this purpose, multipotential stromal cells derived either from subcutaneous human adipose tissue (hAT-MSCs) or from induced pluripotent stem cells (iPSCs) generated from human foreskin fibroblasts (hiPSC-MSCs) were cultured on the biomaterial for 21 days in vitro to generate a 3D bioartifical ACL graft. Stem cell differentiation towards bone and ligament at the ends and central part of the biomaterial was selectively induced using either BMP-2/FGF-2 or TGF-β/FGF-2 combinations, respectively. The bioartificial ACL graft was subsequently implanted in a swine ACL rupture model in place of the surgically removed normal ACL. Four months post-implantation, the tissue engineered ACL graft generated an ACL-like tissue exhibiting morphological and biochemical characteristics resembling those of normal ACL.
KW - human induced pluripotent stem cells
KW - bioartificial ACL graft
KW - tissue engineering
KW - biomaterials
KW - Anterior cruciate ligament reconstruction
KW - Human adipose tissue multipotential stromal cells
U2 - 10.1016/j.scr.2016.04.016
DO - 10.1016/j.scr.2016.04.016
M3 - Article
SN - 1873-5061
JO - Stem Cell Research
JF - Stem Cell Research
ER -