Generation of human induced pluripotent stem cells in defined, feeder-free conditions

Athena Kyrkou, Dimitris Stellas, Marika Syrrou, Apostolos Klinakis, Theodore Fotsis, Carol Murphy

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
222 Downloads (Pure)

Abstract

Herein, we describe a modified protocol for the generation of human induced pluripotent stem cells (hiPS) and expansion under defined, serum free and feeder free conditions. These cells exhibit a high level of plasticity towards various differentiation pathways both in vitro and in vivo. Ultimately, hiPS-derived lines
achieved high standards of three dimensional differentiations on biomaterial scaffolds and promoted in vivo regeneration of complex organs, such as Anterior Cruciate Ligament (in swine ACL-rupture models) and other tissues as well.
Original languageEnglish
JournalStem Cell Research
Early online date12 May 2016
DOIs
Publication statusE-pub ahead of print - 12 May 2016

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