Gemcitabine does not prevent acute rejection of the transplanted liver in rats

Hynek Mergental; Jan Kriz; Eva Honsova; Michal Kudla; Tomas Pantoflicek; Katerina Tcherentsova; Matej Kocik; Frantisek Saudek; Miroslav Ryska

doi:10.1007/s00147-004-0752-1

Gemcitabine does not prevent acute rejection of the transplanted liver in rats

Hynek Mergental, Jan Kriz, Eva Honsova, Michal Kudla, Tomas Pantoflicek, Katerina Tcherentsova, Matej Kocik, Frantisek Saudek, Miroslav Ryska

Immunology and Immunotherapy

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Our study was designed to determine effect of gemcitabine on acute rejection of liver in rats. Liver transplantation was performed in rats of the Dark Agouti (DA) and Lewis (LEW) strains. Recipients were divided into three groups: A, DA-to-LEW without immunosuppression; B, DA-to-LEW, treated with cyclosporine A; C, DA-to-LEW, treated with gemcitabine. Immunosuppressants were subcutaneously injected for seven consecutive days after transplantation. On day 7, blood samples and liver graft tissue specimens were harvested. Group A showed severe rejection changes (RAI 8/9); in group B no rejection changes were present (RAI 0/9), and in group C moderate rejection changes were observed (RAI 6/9). Differences were significant between B vs C and A vs C groups; P<0.05. Serum creatinine and urea levels in the gemcitabine group were significantly lower than those in the cyclosporine A group. We did not confirm gemcitabine ability to prevent liver allograft rejection.

Original language	English
Pages (from-to)	687-91
Number of pages	5
Journal	Transplant international
Volume	17
Issue number	11
DOIs	https://doi.org/10.1007/s00147-004-0752-1
Publication status	Published - Jan 2005

Keywords

Acute Disease
Animals
Creatinine
Cyclosporine
Deoxycytidine
Graft Rejection
Immunosuppressive Agents
Liver
Liver Transplantation
Male
Rats
Rats, Inbred Lew
Rats, Inbred Strains
Treatment Failure
Urea

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10.1007/s00147-004-0752-1

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title = "Gemcitabine does not prevent acute rejection of the transplanted liver in rats",

abstract = "Our study was designed to determine effect of gemcitabine on acute rejection of liver in rats. Liver transplantation was performed in rats of the Dark Agouti (DA) and Lewis (LEW) strains. Recipients were divided into three groups: A, DA-to-LEW without immunosuppression; B, DA-to-LEW, treated with cyclosporine A; C, DA-to-LEW, treated with gemcitabine. Immunosuppressants were subcutaneously injected for seven consecutive days after transplantation. On day 7, blood samples and liver graft tissue specimens were harvested. Group A showed severe rejection changes (RAI 8/9); in group B no rejection changes were present (RAI 0/9), and in group C moderate rejection changes were observed (RAI 6/9). Differences were significant between B vs C and A vs C groups; P<0.05. Serum creatinine and urea levels in the gemcitabine group were significantly lower than those in the cyclosporine A group. We did not confirm gemcitabine ability to prevent liver allograft rejection.",

keywords = "Acute Disease, Animals, Creatinine, Cyclosporine, Deoxycytidine, Graft Rejection, Immunosuppressive Agents, Liver, Liver Transplantation, Male, Rats, Rats, Inbred Lew, Rats, Inbred Strains, Treatment Failure, Urea",

author = "Hynek Mergental and Jan Kriz and Eva Honsova and Michal Kudla and Tomas Pantoflicek and Katerina Tcherentsova and Matej Kocik and Frantisek Saudek and Miroslav Ryska",

year = "2005",

month = jan,

doi = "10.1007/s00147-004-0752-1",

language = "English",

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pages = "687--91",

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T1 - Gemcitabine does not prevent acute rejection of the transplanted liver in rats

AU - Mergental, Hynek

AU - Kriz, Jan

AU - Honsova, Eva

AU - Kudla, Michal

AU - Pantoflicek, Tomas

AU - Tcherentsova, Katerina

AU - Kocik, Matej

AU - Saudek, Frantisek

AU - Ryska, Miroslav

PY - 2005/1

Y1 - 2005/1

N2 - Our study was designed to determine effect of gemcitabine on acute rejection of liver in rats. Liver transplantation was performed in rats of the Dark Agouti (DA) and Lewis (LEW) strains. Recipients were divided into three groups: A, DA-to-LEW without immunosuppression; B, DA-to-LEW, treated with cyclosporine A; C, DA-to-LEW, treated with gemcitabine. Immunosuppressants were subcutaneously injected for seven consecutive days after transplantation. On day 7, blood samples and liver graft tissue specimens were harvested. Group A showed severe rejection changes (RAI 8/9); in group B no rejection changes were present (RAI 0/9), and in group C moderate rejection changes were observed (RAI 6/9). Differences were significant between B vs C and A vs C groups; P<0.05. Serum creatinine and urea levels in the gemcitabine group were significantly lower than those in the cyclosporine A group. We did not confirm gemcitabine ability to prevent liver allograft rejection.

AB - Our study was designed to determine effect of gemcitabine on acute rejection of liver in rats. Liver transplantation was performed in rats of the Dark Agouti (DA) and Lewis (LEW) strains. Recipients were divided into three groups: A, DA-to-LEW without immunosuppression; B, DA-to-LEW, treated with cyclosporine A; C, DA-to-LEW, treated with gemcitabine. Immunosuppressants were subcutaneously injected for seven consecutive days after transplantation. On day 7, blood samples and liver graft tissue specimens were harvested. Group A showed severe rejection changes (RAI 8/9); in group B no rejection changes were present (RAI 0/9), and in group C moderate rejection changes were observed (RAI 6/9). Differences were significant between B vs C and A vs C groups; P<0.05. Serum creatinine and urea levels in the gemcitabine group were significantly lower than those in the cyclosporine A group. We did not confirm gemcitabine ability to prevent liver allograft rejection.

KW - Acute Disease

KW - Animals

KW - Creatinine

KW - Cyclosporine

KW - Deoxycytidine

KW - Graft Rejection

KW - Immunosuppressive Agents

KW - Liver

KW - Liver Transplantation

KW - Male

KW - Rats

KW - Rats, Inbred Lew

KW - Rats, Inbred Strains

KW - Treatment Failure

KW - Urea

U2 - 10.1007/s00147-004-0752-1

DO - 10.1007/s00147-004-0752-1

M3 - Article

C2 - 15580336

SN - 0934-0874

VL - 17

SP - 687

EP - 691

JO - Transplant international

JF - Transplant international

IS - 11

ER -

Gemcitabine does not prevent acute rejection of the transplanted liver in rats

Abstract

Keywords

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