Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies

Isabel Fofana; Laetitia Zona; Christine Thumann; Laura Heydmann; Sarah C Durand; Joachim Lupberger; Hubert E Blum; Patrick Pessaux; Claire Gondeau; Gary M Reynolds; Jane A McKeating; Fritz Grunert; John Thompson; Mirjam B Zeisel; Thomas F Baumert

doi:10.1128/JVI.01691-13

Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies

Isabel Fofana, Laetitia Zona, Christine Thumann, Laura Heydmann, Sarah C Durand, Joachim Lupberger, Hubert E Blum, Patrick Pessaux, Claire Gondeau, Gary M Reynolds, Jane A McKeating, Fritz Grunert, John Thompson, Mirjam B Zeisel, Thomas F Baumert

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The relevance of claudin-6 and claudin-9 in hepatitis C virus (HCV) entry remains elusive. We produced claudin-6- or claudin-9-specific monoclonal antibodies that inhibit HCV entry into nonhepatic cells expressing exogenous claudin-6 or claudin-9. These antibodies had no effect on HCV infection of hepatoma cells or primary hepatocytes. Thus, although claudin-6 and claudin-9 can serve as entry factors in cell lines, HCV infection into human hepatocytes is not dependent on claudin-6 and claudin-9.

Original language	English
Pages (from-to)	10405-10
Number of pages	6
Journal	Journal of virology
Volume	87
Issue number	18
DOIs	https://doi.org/10.1128/JVI.01691-13
Publication status	Published - Sept 2013

Keywords

Antibodies, Monoclonal
Cells, Cultured
Claudins
Hepacivirus
Hepatocytes
Humans
Virus Internalization

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JVI.01691-13

The Role of Hepatitis C Virus Glycoprotein-Receptor Polymorphism in Viral Pathogenesis
McKeating, J. (Principal Investigator)
Medical Research Council
1/01/12 → 30/06/17
Project: Research Councils

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Fofana, I., Zona, L., Thumann, C., Heydmann, L., Durand, S. C., Lupberger, J., Blum, H. E., Pessaux, P., Gondeau, C., Reynolds, G. M., McKeating, J. A., Grunert, F., Thompson, J., Zeisel, M. B., & Baumert, T. F. (2013). Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies. Journal of virology, 87(18), 10405-10. https://doi.org/10.1128/JVI.01691-13

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title = "Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies",

abstract = "The relevance of claudin-6 and claudin-9 in hepatitis C virus (HCV) entry remains elusive. We produced claudin-6- or claudin-9-specific monoclonal antibodies that inhibit HCV entry into nonhepatic cells expressing exogenous claudin-6 or claudin-9. These antibodies had no effect on HCV infection of hepatoma cells or primary hepatocytes. Thus, although claudin-6 and claudin-9 can serve as entry factors in cell lines, HCV infection into human hepatocytes is not dependent on claudin-6 and claudin-9.",

keywords = "Antibodies, Monoclonal, Cells, Cultured, Claudins, Hepacivirus, Hepatocytes, Humans, Virus Internalization",

author = "Isabel Fofana and Laetitia Zona and Christine Thumann and Laura Heydmann and Durand, \{Sarah C\} and Joachim Lupberger and Blum, \{Hubert E\} and Patrick Pessaux and Claire Gondeau and Reynolds, \{Gary M\} and McKeating, \{Jane A\} and Fritz Grunert and John Thompson and Zeisel, \{Mirjam B\} and Baumert, \{Thomas F\}",

year = "2013",

month = sep,

doi = "10.1128/JVI.01691-13",

language = "English",

volume = "87",

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Fofana, I, Zona, L, Thumann, C, Heydmann, L, Durand, SC, Lupberger, J, Blum, HE, Pessaux, P, Gondeau, C, Reynolds, GM, McKeating, JA, Grunert, F, Thompson, J, Zeisel, MB & Baumert, TF 2013, 'Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies', Journal of virology, vol. 87, no. 18, pp. 10405-10. https://doi.org/10.1128/JVI.01691-13

TY - JOUR

T1 - Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies

AU - Fofana, Isabel

AU - Zona, Laetitia

AU - Thumann, Christine

AU - Heydmann, Laura

AU - Durand, Sarah C

AU - Lupberger, Joachim

AU - Blum, Hubert E

AU - Pessaux, Patrick

AU - Gondeau, Claire

AU - Reynolds, Gary M

AU - McKeating, Jane A

AU - Grunert, Fritz

AU - Thompson, John

AU - Zeisel, Mirjam B

AU - Baumert, Thomas F

PY - 2013/9

Y1 - 2013/9

N2 - The relevance of claudin-6 and claudin-9 in hepatitis C virus (HCV) entry remains elusive. We produced claudin-6- or claudin-9-specific monoclonal antibodies that inhibit HCV entry into nonhepatic cells expressing exogenous claudin-6 or claudin-9. These antibodies had no effect on HCV infection of hepatoma cells or primary hepatocytes. Thus, although claudin-6 and claudin-9 can serve as entry factors in cell lines, HCV infection into human hepatocytes is not dependent on claudin-6 and claudin-9.

AB - The relevance of claudin-6 and claudin-9 in hepatitis C virus (HCV) entry remains elusive. We produced claudin-6- or claudin-9-specific monoclonal antibodies that inhibit HCV entry into nonhepatic cells expressing exogenous claudin-6 or claudin-9. These antibodies had no effect on HCV infection of hepatoma cells or primary hepatocytes. Thus, although claudin-6 and claudin-9 can serve as entry factors in cell lines, HCV infection into human hepatocytes is not dependent on claudin-6 and claudin-9.

KW - Antibodies, Monoclonal

KW - Cells, Cultured

KW - Claudins

KW - Hepacivirus

KW - Hepatocytes

KW - Humans

KW - Virus Internalization

U2 - 10.1128/JVI.01691-13

DO - 10.1128/JVI.01691-13

M3 - Article

C2 - 23864633

SN - 0022-538X

VL - 87

SP - 10405

EP - 10410

JO - Journal of virology

JF - Journal of virology

IS - 18

ER -

Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies

Abstract

Keywords

UN SDGs

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The Role of Hepatitis C Virus Glycoprotein-Receptor Polymorphism in Viral Pathogenesis

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