Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies

Isabel Fofana; Laetitia Zona; Christine Thumann; Laura Heydmann; Sarah C Durand; Joachim Lupberger; Hubert E Blum; Patrick Pessaux; Claire Gondeau; Gary M Reynolds; Jane A McKeating; Fritz Grunert; John Thompson; Mirjam B Zeisel; Thomas F Baumert

doi:10.1128/JVI.01691-13

Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies

Isabel Fofana, Laetitia Zona, Christine Thumann, Laura Heydmann, Sarah C Durand, Joachim Lupberger, Hubert E Blum, Patrick Pessaux, Claire Gondeau, Gary M Reynolds, Jane A McKeating, Fritz Grunert, John Thompson, Mirjam B Zeisel, Thomas F Baumert

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The relevance of claudin-6 and claudin-9 in hepatitis C virus (HCV) entry remains elusive. We produced claudin-6- or claudin-9-specific monoclonal antibodies that inhibit HCV entry into nonhepatic cells expressing exogenous claudin-6 or claudin-9. These antibodies had no effect on HCV infection of hepatoma cells or primary hepatocytes. Thus, although claudin-6 and claudin-9 can serve as entry factors in cell lines, HCV infection into human hepatocytes is not dependent on claudin-6 and claudin-9.

Original language	English
Pages (from-to)	10405-10
Number of pages	6
Journal	Journal of virology
Volume	87
Issue number	18
DOIs	https://doi.org/10.1128/JVI.01691-13
Publication status	Published - Sept 2013

Keywords

Antibodies, Monoclonal
Cells, Cultured
Claudins
Hepacivirus
Hepatocytes
Humans
Virus Internalization

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JVI.01691-13

The Role of Hepatitis C Virus Glycoprotein-Receptor Polymorphism in Viral Pathogenesis
McKeating, J.
Medical Research Council
1/01/12 → 30/06/17
Project: Research Councils

Cite this

Fofana, I., Zona, L., Thumann, C., Heydmann, L., Durand, S. C., Lupberger, J., Blum, H. E., Pessaux, P., Gondeau, C., Reynolds, G. M., McKeating, J. A., Grunert, F., Thompson, J., Zeisel, M. B., & Baumert, T. F. (2013). Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies. Journal of virology, 87(18), 10405-10. https://doi.org/10.1128/JVI.01691-13

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title = "Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies",

abstract = "The relevance of claudin-6 and claudin-9 in hepatitis C virus (HCV) entry remains elusive. We produced claudin-6- or claudin-9-specific monoclonal antibodies that inhibit HCV entry into nonhepatic cells expressing exogenous claudin-6 or claudin-9. These antibodies had no effect on HCV infection of hepatoma cells or primary hepatocytes. Thus, although claudin-6 and claudin-9 can serve as entry factors in cell lines, HCV infection into human hepatocytes is not dependent on claudin-6 and claudin-9.",

keywords = "Antibodies, Monoclonal, Cells, Cultured, Claudins, Hepacivirus, Hepatocytes, Humans, Virus Internalization",

author = "Isabel Fofana and Laetitia Zona and Christine Thumann and Laura Heydmann and Durand, {Sarah C} and Joachim Lupberger and Blum, {Hubert E} and Patrick Pessaux and Claire Gondeau and Reynolds, {Gary M} and McKeating, {Jane A} and Fritz Grunert and John Thompson and Zeisel, {Mirjam B} and Baumert, {Thomas F}",

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doi = "10.1128/JVI.01691-13",

language = "English",

volume = "87",

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Fofana, I, Zona, L, Thumann, C, Heydmann, L, Durand, SC, Lupberger, J, Blum, HE, Pessaux, P, Gondeau, C, Reynolds, GM, McKeating, JA, Grunert, F, Thompson, J, Zeisel, MB & Baumert, TF 2013, 'Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies', Journal of virology, vol. 87, no. 18, pp. 10405-10. https://doi.org/10.1128/JVI.01691-13

TY - JOUR

T1 - Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies

AU - Fofana, Isabel

AU - Zona, Laetitia

AU - Thumann, Christine

AU - Heydmann, Laura

AU - Durand, Sarah C

AU - Lupberger, Joachim

AU - Blum, Hubert E

AU - Pessaux, Patrick

AU - Gondeau, Claire

AU - Reynolds, Gary M

AU - McKeating, Jane A

AU - Grunert, Fritz

AU - Thompson, John

AU - Zeisel, Mirjam B

AU - Baumert, Thomas F

PY - 2013/9

Y1 - 2013/9

N2 - The relevance of claudin-6 and claudin-9 in hepatitis C virus (HCV) entry remains elusive. We produced claudin-6- or claudin-9-specific monoclonal antibodies that inhibit HCV entry into nonhepatic cells expressing exogenous claudin-6 or claudin-9. These antibodies had no effect on HCV infection of hepatoma cells or primary hepatocytes. Thus, although claudin-6 and claudin-9 can serve as entry factors in cell lines, HCV infection into human hepatocytes is not dependent on claudin-6 and claudin-9.

AB - The relevance of claudin-6 and claudin-9 in hepatitis C virus (HCV) entry remains elusive. We produced claudin-6- or claudin-9-specific monoclonal antibodies that inhibit HCV entry into nonhepatic cells expressing exogenous claudin-6 or claudin-9. These antibodies had no effect on HCV infection of hepatoma cells or primary hepatocytes. Thus, although claudin-6 and claudin-9 can serve as entry factors in cell lines, HCV infection into human hepatocytes is not dependent on claudin-6 and claudin-9.

KW - Antibodies, Monoclonal

KW - Cells, Cultured

KW - Claudins

KW - Hepacivirus

KW - Hepatocytes

KW - Humans

KW - Virus Internalization

U2 - 10.1128/JVI.01691-13

DO - 10.1128/JVI.01691-13

M3 - Article

C2 - 23864633

SN - 0022-538X

VL - 87

SP - 10405

EP - 10410

JO - Journal of virology

JF - Journal of virology

IS - 18

ER -

Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies

Abstract

Keywords

UN SDGs

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The Role of Hepatitis C Virus Glycoprotein-Receptor Polymorphism in Viral Pathogenesis

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