Fluoroquinolone resistance does not impose a cost on the fitness of Clostridium difficile in vitro

François Wasels; Sarah Kuehne; Stephen T Cartman; Patrizia Spigaglia; Fabrizio Barbanti; Nigel P Minton; Paola Mastrantonio

doi:10.1128/AAC.04503-14

Fluoroquinolone resistance does not impose a cost on the fitness of Clostridium difficile in vitro

François Wasels
, Sarah Kuehne
, Stephen T Cartman
, Patrizia Spigaglia
, Fabrizio Barbanti
, Nigel P Minton
, Paola Mastrantonio

Dentistry

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Point mutations conferring resistance to fluoroquinolones were introduced in the gyr genes of the reference strain Clostridium difficile 630. Only mutants with the substitution Thr-82→Ile in GyrA, which characterizes the hypervirulent epidemic clone III/027/NAP1, were resistant to all fluoroquinolones tested. The absence of a fitness cost in vitro for the most frequent mutations detected in resistant clinical isolates suggests that resistance will be maintained even in the absence of antibiotic pressure.

Original language	English
Pages (from-to)	1794-1796
Number of pages	3
Journal	Antimicrobial Agents and Chemotherapy
Volume	59
Issue number	3
Early online date	22 Dec 2014
DOIs	https://doi.org/10.1128/AAC.04503-14
Publication status	Published - Mar 2015

Keywords

Anti-Bacterial Agents
Clostridium difficile
DNA Gyrase
Drug Resistance, Bacterial
Fluoroquinolones
Point Mutation
Journal Article
Research Support, Non-U.S. Gov't

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title = "Fluoroquinolone resistance does not impose a cost on the fitness of Clostridium difficile in vitro",

abstract = "Point mutations conferring resistance to fluoroquinolones were introduced in the gyr genes of the reference strain Clostridium difficile 630. Only mutants with the substitution Thr-82→Ile in GyrA, which characterizes the hypervirulent epidemic clone III/027/NAP1, were resistant to all fluoroquinolones tested. The absence of a fitness cost in vitro for the most frequent mutations detected in resistant clinical isolates suggests that resistance will be maintained even in the absence of antibiotic pressure.",

keywords = "Anti-Bacterial Agents, Clostridium difficile, DNA Gyrase, Drug Resistance, Bacterial, Fluoroquinolones, Point Mutation, Journal Article, Research Support, Non-U.S. Gov't",

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language = "English",

volume = "59",

pages = "1794--1796",

journal = "Antimicrobial Agents and Chemotherapy",

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AU - Wasels, François

AU - Kuehne, Sarah

AU - Cartman, Stephen T

AU - Spigaglia, Patrizia

AU - Barbanti, Fabrizio

AU - Minton, Nigel P

AU - Mastrantonio, Paola

PY - 2015/3

Y1 - 2015/3

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AB - Point mutations conferring resistance to fluoroquinolones were introduced in the gyr genes of the reference strain Clostridium difficile 630. Only mutants with the substitution Thr-82→Ile in GyrA, which characterizes the hypervirulent epidemic clone III/027/NAP1, were resistant to all fluoroquinolones tested. The absence of a fitness cost in vitro for the most frequent mutations detected in resistant clinical isolates suggests that resistance will be maintained even in the absence of antibiotic pressure.

KW - Anti-Bacterial Agents

KW - Clostridium difficile

KW - DNA Gyrase

KW - Drug Resistance, Bacterial

KW - Fluoroquinolones

KW - Point Mutation

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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DO - 10.1128/AAC.04503-14

M3 - Article

C2 - 25534738

SN - 0066-4804

VL - 59

SP - 1794

EP - 1796

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 3

ER -

Fluoroquinolone resistance does not impose a cost on the fitness of Clostridium difficile in vitro

Abstract

Keywords

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