TY - JOUR
T1 - Fluorescent PSC-derived cardiomyocyte reporter lines
T2 - generation approaches and their applications in cardiovascular medicine
AU - Sontayananon, Naeramit
AU - Redwood, Charles
AU - Davies, Benjamin
AU - Gehmlich, Katja
PY - 2020/11/16
Y1 - 2020/11/16
N2 - Recent advances have made pluripotent stem cell (PSC)-derived cardiomyocytes an attractive option to model both normal and diseased cardiac function at the single-cell level. However, in vitro differentiation yields heterogeneous populations of cardiomyocytes and other cell types, potentially confounding phenotypic analyses. Fluorescent PSC-derived cardiomyocyte reporter systems allow specific cell lineages to be labelled, facilitating cell isolation for downstream applications including drug testing, disease modelling and cardiac regeneration. In this review, the different genetic strategies used to generate such reporter lines are presented with an emphasis on their relative technical advantages and disadvantages. Next, we explore how the fluorescent reporter lines have provided insights into cardiac development and cardiomyocyte physiology. Finally, we discuss how exciting new approaches using PSC-derived cardiomyocyte reporter lines are contributing to progress in cardiac cell therapy with respect to both graft adaptation and clinical safety.
AB - Recent advances have made pluripotent stem cell (PSC)-derived cardiomyocytes an attractive option to model both normal and diseased cardiac function at the single-cell level. However, in vitro differentiation yields heterogeneous populations of cardiomyocytes and other cell types, potentially confounding phenotypic analyses. Fluorescent PSC-derived cardiomyocyte reporter systems allow specific cell lineages to be labelled, facilitating cell isolation for downstream applications including drug testing, disease modelling and cardiac regeneration. In this review, the different genetic strategies used to generate such reporter lines are presented with an emphasis on their relative technical advantages and disadvantages. Next, we explore how the fluorescent reporter lines have provided insights into cardiac development and cardiomyocyte physiology. Finally, we discuss how exciting new approaches using PSC-derived cardiomyocyte reporter lines are contributing to progress in cardiac cell therapy with respect to both graft adaptation and clinical safety.
KW - CM purification
KW - cardiac development
KW - fluorescent PSC-CM reporter lines
KW - optical electrophysiology
KW - transgenic methods
UR - http://www.scopus.com/inward/record.url?scp=85096057107&partnerID=8YFLogxK
U2 - 10.3390/biology9110402
DO - 10.3390/biology9110402
M3 - Review article
SN - 2079-7737
VL - 9
SP - 1
EP - 24
JO - Biology
JF - Biology
IS - 11
M1 - 402
ER -