Fluorescent Nucleic Acid Probes in Living Cells

Anne Seefeld; G. R. Kokil; K. R. Tupally; Harendra S. Parekh; Dirk-Peter Herten; Ganesh R. Kokil; Karnaker R. Tupally

doi:10.1007/978-3-642-36853-0_12

Fluorescent Nucleic Acid Probes in Living Cells

Anne Seefeld, G. R. Kokil, K. R. Tupally, Harendra S. Parekh, Dirk-Peter Herten, Ganesh R. Kokil, Karnaker R. Tupally

Cardiovascular Sciences

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Abstract

During the last decade a variety of fluorescent probes have been developed that allow quantitative analysis of specific DNA and RNA sequences in biological samples. In combination with recently developed methods in fluorescence microscopy, this offers the unique opportunity to quantitatively study DNA and RNA content in fixed and living cells in response to cellular stimulation. This chapter is intended to supply the reader with an overview of relevant approaches for probing specific DNA and RNA sequences in biological samples with a focus on DNA hairpin probes. We follow this with discussion on the modes of cellular entry from the perspective of both viral and non-viral carriers. To conclude we elaborate on the key barriers that need to be overcome for successful transfer of DNA probes into living cells.

Original language	English
Title of host publication	DNA and RNA Nanobiotechnologies in Medicine: Diagnosis and Treatment of Diseases
Chapter	291-328
DOIs	https://doi.org/10.1007/978-3-642-36853-0_12
Publication status	Published - 1 Jan 2013

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title = "Fluorescent Nucleic Acid Probes in Living Cells",

abstract = "During the last decade a variety of fluorescent probes have been developed that allow quantitative analysis of specific DNA and RNA sequences in biological samples. In combination with recently developed methods in fluorescence microscopy, this offers the unique opportunity to quantitatively study DNA and RNA content in fixed and living cells in response to cellular stimulation. This chapter is intended to supply the reader with an overview of relevant approaches for probing specific DNA and RNA sequences in biological samples with a focus on DNA hairpin probes. We follow this with discussion on the modes of cellular entry from the perspective of both viral and non-viral carriers. To conclude we elaborate on the key barriers that need to be overcome for successful transfer of DNA probes into living cells.",

author = "Anne Seefeld and Kokil, {G. R.} and Tupally, {K. R.} and Parekh, {Harendra S.} and Dirk-Peter Herten and Kokil, {Ganesh R.} and Tupally, {Karnaker R.}",

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AU - Seefeld, Anne

AU - Kokil, G. R.

AU - Tupally, K. R.

AU - Parekh, Harendra S.

AU - Herten, Dirk-Peter

AU - Kokil, Ganesh R.

AU - Tupally, Karnaker R.

PY - 2013/1/1

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AB - During the last decade a variety of fluorescent probes have been developed that allow quantitative analysis of specific DNA and RNA sequences in biological samples. In combination with recently developed methods in fluorescence microscopy, this offers the unique opportunity to quantitatively study DNA and RNA content in fixed and living cells in response to cellular stimulation. This chapter is intended to supply the reader with an overview of relevant approaches for probing specific DNA and RNA sequences in biological samples with a focus on DNA hairpin probes. We follow this with discussion on the modes of cellular entry from the perspective of both viral and non-viral carriers. To conclude we elaborate on the key barriers that need to be overcome for successful transfer of DNA probes into living cells.

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DO - 10.1007/978-3-642-36853-0_12

M3 - Chapter

BT - DNA and RNA Nanobiotechnologies in Medicine: Diagnosis and Treatment of Diseases

ER -

Fluorescent Nucleic Acid Probes in Living Cells

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