Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis

James E Scriven; Lisa M Graham; Charlotte Schutz; Thomas J Scriba; Robert J Wilkinson; David R Boulware; Graeme Meintjes; David G Lalloo; Britta C Urban

doi:10.1128/JCM.03002-15

Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis

James E Scriven
, Lisa M Graham
, Charlotte Schutz
, Thomas J Scriba
, Robert J Wilkinson
, David R Boulware
, Graeme Meintjes
, David G Lalloo
, Britta C Urban

Microbes, Infection and Microbiomes

Research output: Contribution to journal › Article › peer-review

Abstract

Fungal burden in the cerebrospinal fluid is an important determinant of mortality in cryptococcal meningitis, but its use in aiding clinical decision making is hampered by the time involved to perform quantitative cultures. Here, we demonstrate the potential of flow cytometry as a novel and rapid technique to address this issue.

Original language	English
Pages (from-to)	802-4
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	54
Issue number	3
DOIs	https://doi.org/10.1128/JCM.03002-15
Publication status	Published - Mar 2016

Bibliographical note

Keywords

Cerebrospinal Fluid/microbiology
Colony Count, Microbial/methods
Cryptococcus neoformans/isolation & purification
Flow Cytometry/methods
Humans
Meningitis, Cryptococcal/diagnosis

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10.1128/JCM.03002-15

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title = "Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis",

abstract = "Fungal burden in the cerebrospinal fluid is an important determinant of mortality in cryptococcal meningitis, but its use in aiding clinical decision making is hampered by the time involved to perform quantitative cultures. Here, we demonstrate the potential of flow cytometry as a novel and rapid technique to address this issue.",

keywords = "Cerebrospinal Fluid/microbiology, Colony Count, Microbial/methods, Cryptococcus neoformans/isolation \& purification, Flow Cytometry/methods, Humans, Meningitis, Cryptococcal/diagnosis",

author = "Scriven, \{James E\} and Graham, \{Lisa M\} and Charlotte Schutz and Scriba, \{Thomas J\} and Wilkinson, \{Robert J\} and Boulware, \{David R\} and Graeme Meintjes and Lalloo, \{David G\} and Urban, \{Britta C\}",

note = "Copyright {\textcopyright} 2016 Scriven et al.",

year = "2016",

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doi = "10.1128/JCM.03002-15",

language = "English",

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pages = "802--4",

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T1 - Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis

AU - Scriven, James E

AU - Graham, Lisa M

AU - Schutz, Charlotte

AU - Scriba, Thomas J

AU - Wilkinson, Robert J

AU - Boulware, David R

AU - Meintjes, Graeme

AU - Lalloo, David G

AU - Urban, Britta C

PY - 2016/3

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N2 - Fungal burden in the cerebrospinal fluid is an important determinant of mortality in cryptococcal meningitis, but its use in aiding clinical decision making is hampered by the time involved to perform quantitative cultures. Here, we demonstrate the potential of flow cytometry as a novel and rapid technique to address this issue.

AB - Fungal burden in the cerebrospinal fluid is an important determinant of mortality in cryptococcal meningitis, but its use in aiding clinical decision making is hampered by the time involved to perform quantitative cultures. Here, we demonstrate the potential of flow cytometry as a novel and rapid technique to address this issue.

KW - Cerebrospinal Fluid/microbiology

KW - Colony Count, Microbial/methods

KW - Cryptococcus neoformans/isolation & purification

KW - Flow Cytometry/methods

KW - Humans

KW - Meningitis, Cryptococcal/diagnosis

U2 - 10.1128/JCM.03002-15

DO - 10.1128/JCM.03002-15

M3 - Article

C2 - 26719441

SN - 0095-1137

VL - 54

SP - 802

EP - 804

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 3

ER -

Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis

Abstract

Bibliographical note

Keywords

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