Flow Cytometry of Yeasts and Other Fungi

Flow cytometry is used extensively to investigate many aspects of yeast physiology and cell biology. This chapter describes the most common applications of flow cytometry and fluorescence-activated cell sorting (FACS) to yeasts and other fungi, and reviews recent studies to capture current directions in the field. Pure and applied aspects are discussed, with a focus on the areas of fundamental cell biology, medical applications, monitoring during bioprocesses including brewing, and the use of yeast as a molecular biology tool. Novel flow cytometric and microfluidic systems which utilise yeast as a model organism are also described and their relevance to previous research in the area and their possible application for future fundamental and applied research are discussed.