Flow cytometric analysis of CD4+ T cell re-activation following anti-PD1 immunotherapy in a transgenic mouse model

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Abstract

This protocol uses the Tg4 Nr4a3-Tocky mouse model to recalibrate T cell activation thresholds and reveals the role that immune checkpoints play in controlling T cell activation. The example approach here uses flow cytometry to characterize quantitative and qualitative changes in splenic CD4+ T cells reactivated in the presence of anti-PD1 immunotherapy. The protocol is optimized for studying anti-PD1 pathway blockade only. The protocol is not compatible with cellular fixation, and T cells should be analyzed immediately after staining.

For complete details on the use and execution of this protocol, please refer to Elliot et al. (2021).
Original languageEnglish
Article number101161
JournalSTAR Protocols
Volume3
Issue number1
Early online date3 Feb 2022
DOIs
Publication statusPublished - 18 Mar 2022

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