FibroScan-AST (FAST) score for the non-invasive identification of patients with non-alcoholic steatohepatitis with significant activity and fibrosis: a prospective derivation and global validation study

Philip Newsome, M Sasso, Jon Deeks, A Paredes, J Boursier, WK Chan, Y Yilmaz, S Czernichow, MH Zheng, VW Wong, M Allison, E Tsochatzis, QM Anstee, David A Sheridan, Peter J Eddowes, Indra N Guha, Jeremy F Cobbold, Valérie Paradis, Pierre Bedossa, Véronique MietteCéline Fournier-Poizat, Laurent Sandrin, Stephen A Harison

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Abstract

Background
The burden of non-alcoholic fatty liver disease (NAFLD) is increasing globally, and a major priority is to identify patients with non-alcoholic steatohepatitis (NASH) who are at greater risk of progression to cirrhosis, and who will be candidates for clinical trials and emerging new pharmacotherapies. We aimed to develop a score to identify patients with NASH, elevated NAFLD activity score (NAS≥4), and advanced fibrosis (stage 2 or higher [F≥2]).

Methods
This prospective study included a derivation cohort before validation in multiple international cohorts. The derivation cohort was a cross-sectional, multicentre study of patients aged 18 years or older, scheduled to have a liver biopsy for suspicion of NAFLD at seven tertiary care liver centres in England. This was a prespecified secondary outcome of a study for which the primary endpoints have already been reported. Liver stiffness measurement (LSM) by vibration-controlled transient elastography and controlled attenuation parameter (CAP) measured by FibroScan device were combined with aspartate aminotransferase (AST), alanine aminotransferase (ALT), or AST:ALT ratio. To identify those patients with NASH, an elevated NAS, and significant fibrosis, the best fitting multivariable logistic regression model was identified and internally validated using boot-strapping. Score calibration and discrimination performance were determined in both the derivation dataset in England, and seven independent international (France, USA, China, Malaysia, Turkey) histologically confirmed cohorts of patients with NAFLD (external validation cohorts). This study is registered with ClinicalTrials.gov, number NCT01985009.

Findings
Between March 20, 2014, and Jan 17, 2017, 350 patients with suspected NAFLD attending liver clinics in England were prospectively enrolled in the derivation cohort. The most predictive model combined LSM, CAP, and AST, and was designated FAST (FibroScan-AST). Performance was satisfactory in the derivation dataset (C-statistic 0·80, 95% CI 0·76–0·85) and was well calibrated. In external validation cohorts, calibration of the score was satisfactory and discrimination was good across the full range of validation cohorts (C-statistic range 0·74–0·95, 0·85; 95% CI 0·83–0·87 in the pooled external validation patients' cohort; n=1026). Cutoff was 0·35 for sensitivity of 0·90 or greater and 0·67 for specificity of 0·90 or greater in the derivation cohort, leading to a positive predictive value (PPV) of 0·83 (84/101) and a negative predictive value (NPV) of 0·85 (93/110). In the external validation cohorts, PPV ranged from 0·33 to 0·81 and NPV from 0·73 to 1·0.

Interpretation
The FAST score provides an efficient way to non-invasively identify patients at risk of progressive NASH for clinical trials or treatments when they become available, and thereby reduce unnecessary liver biopsy in patients unlikely to have significant disease.

Funding
Echosens and UK National Institute for Health Research.
Original languageEnglish
Pages (from-to)362-373
Number of pages12
JournalThe Lancet Gastroenterology & Hepatology
Volume5
Issue number4
Early online date3 Feb 2020
DOIs
Publication statusPublished - Apr 2020

Keywords

  • Adult
  • Alanine Transaminase/analysis
  • Aspartate Aminotransferases/analysis
  • Biopsy
  • China/epidemiology
  • Cohort Studies
  • Cross-Sectional Studies
  • Disease Progression
  • Elasticity Imaging Techniques/methods
  • England/epidemiology
  • Female
  • Fibrosis/classification
  • France/epidemiology
  • Humans
  • Liver Cirrhosis/epidemiology
  • Liver/metabolism
  • Malaysia/epidemiology
  • Male
  • Middle Aged
  • Non-alcoholic Fatty Liver Disease/complications
  • Predictive Value of Tests
  • Prospective Studies
  • Sensitivity and Specificity
  • Turkey/epidemiology
  • United States/epidemiology

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

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