Fast targeted multidimensional NMR metabolomics of colorectal cancer

Christian Ludwig, Douglas Ward, Ashley Martin, Mark Viant, Tariq Ismail, Philip Johnson, Michael Wakelam, Ulrich Gunther

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The study of small molecules in body fluids has become an important tool to monitor the state of biological organisms. Applications range from model studies using cell lines to applications where human body fluids are used to monitor disease states or drug responses. NMR spectroscopy has been an important tool for metabolomics although severe overlap of signals has limited the number of compounds, which can be unambiguously identified and quantified. Therefore, deconvolution of NMR spectra is one of the greatest challenges for NMR-based metabolomics. This has commonly been achieved by using multidimensional spectra that have the disadvantage of requiring significantly longer acquisition times. Recently, a number of methods have been described to record NMR spectra much faster. Here, we explore the use of Hadamard-encoded TOCSY spectra to simultaneously select multiple lines from crowded NMR spectra of blood serum samples to acquire pseudo-two-dimensional spectra in minutes which would otherwise require many hours. The potential of this approach is demonstrated for the detection of a signature for colorectal cancer from human blood samples. Copyright (C) 2009 John Wiley & Sons, Ltd.
Original languageEnglish
Pages (from-to)n/a-n/a
JournalMagnetic Resonance in Chemistry
Volume47
Issue numberSuppl 1:S68-73
DOIs
Publication statusPublished - 1 Jan 2009

Keywords

  • TOCSY
  • 17 beta-HSD1 NMR
  • metabonomics
  • Epitope mapping
  • Hadamard encoding
  • colorectal cancer
  • NMR
  • nuclear magnetic resonance
  • metabolomics
  • Hydroxysteroid dehydrogenase
  • 17 beta-Hydroxysteroid dehydrogenase type-1

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