TY - JOUR
T1 - Fabrication and characterisation of novel screen-printed tubular microband electrodes, and their application to the measurement of hydrogen peroxide
AU - Rawson, F.J.
AU - Cowell, D.C.
AU - Hart, J.P.
AU - Purcell, W.M.
AU - Xu, J.
AU - Fielden, P.R.
AU - Biddle, N.
PY - 2007/9/1
Y1 - 2007/9/1
N2 - A new method of using screen-printed carbon electrodes (SPCEs) incorporating the electrocatalyst cobalt phthalocyanine (CoPC) for the manufacture of tubular microband electrodes for hydrogen peroxide detection is described. Characterisation of these electrodes using potassium ferrocyanide, with cyclic voltammetry, has shown that steady state behaviour is displayed which is indicative of microelectrode behaviour. The current density obtained from the voltammogram was compared to that obtained for a conventional sized CoPC-SPCE, and the values were 5618 and 35.65 μA cm, respectively. Cyclic voltammetry was carried out for the same electrodes, using 7 mM HO prepared in phosphate buffer at scan rates between 1 and 50 mV s and no significant increase in current response was observed. The application of these tubular microband CoPC-SPCEs, to the measurement of HO using chronoamperometry was investigated. A calibration study was performed and the plot showed a sensitivity value of 252 μA mM cm and a lower detection limit of 70 μM. We have shown that the chronoamperometric current response could be calculated using a modified equation originally developed for a plain microband electrode. This study provides a platform for using screen-printed carbon electrodes for the fabrication of oxidase based microbiosensors, for the determination of a variety of cellular metabolites.
AB - A new method of using screen-printed carbon electrodes (SPCEs) incorporating the electrocatalyst cobalt phthalocyanine (CoPC) for the manufacture of tubular microband electrodes for hydrogen peroxide detection is described. Characterisation of these electrodes using potassium ferrocyanide, with cyclic voltammetry, has shown that steady state behaviour is displayed which is indicative of microelectrode behaviour. The current density obtained from the voltammogram was compared to that obtained for a conventional sized CoPC-SPCE, and the values were 5618 and 35.65 μA cm, respectively. Cyclic voltammetry was carried out for the same electrodes, using 7 mM HO prepared in phosphate buffer at scan rates between 1 and 50 mV s and no significant increase in current response was observed. The application of these tubular microband CoPC-SPCEs, to the measurement of HO using chronoamperometry was investigated. A calibration study was performed and the plot showed a sensitivity value of 252 μA mM cm and a lower detection limit of 70 μM. We have shown that the chronoamperometric current response could be calculated using a modified equation originally developed for a plain microband electrode. This study provides a platform for using screen-printed carbon electrodes for the fabrication of oxidase based microbiosensors, for the determination of a variety of cellular metabolites.
UR - http://www.scopus.com/inward/record.url?partnerID=yv4JPVwI&eid=2-s2.0-34547565536&md5=21bcbaaad3750a4372fdf67fd66f521f
U2 - 10.1016/j.electacta.2007.05.062
DO - 10.1016/j.electacta.2007.05.062
M3 - Article
AN - SCOPUS:34547565536
SN - 0013-4686
VL - 52
SP - 7248
EP - 7253
JO - Electrochimica Acta
JF - Electrochimica Acta
IS - 25
ER -