Projects per year
The dynamic nature of micellar nanostructures is employed to form a self-assembled Förster resonance energy transfer (FRET) nanoplatform for enhanced sensing of DNA. The platform consists of lipid oligonucleotide FRET probes incorporated into micellar scaffolds, where single recognition events result in fusion and fission of DNA mixed micelles, triggering the fluorescence response of multiple rather than a single FRET pair. In comparison to conventional FRET substrates where a single donor interacts with a single acceptor, the micellar multiplex FRET system showed ∼20- and ∼3-fold enhancements in the limit of detection and FRET efficiency, respectively. This supramolecular signal amplification approach could potentially be used to improve FRET-based diagnostic assays of nucleic acid and non-DNA based targets.
|Number of pages||8|
|Early online date||7 May 2021|
|Publication status||Published - 25 May 2021|
Bibliographical noteFunding Information: We acknowledge financial support of this work by the EPSRC (Grant EP/K027263/1) and ERC (Consolidator Grant 614787). This project was also supported by the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant Agreement No. 795415. The Centre for Chemical and Materials Analysis at the University of Birmingham is acknowledged for technical support.
- DNA sensing
- lipid oligonucleotide conjugates
- signal enhancement
- Fluorescence Resonance Energy Transfer
- Nucleic Acids
- Lipid Oligonucleotide Conjugates
- Dna Sensing
- Signal Enhancement
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Materials Science(all)
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- 2 Finished
FP7_ERC - GLYCOSURF
European Commission, European Commission - Management Costs
1/12/14 → 31/05/21
Surface-Based Molecular Imprinting for Glycoprotein Recognition
Fossey, J. & Mendes, P.
Engineering & Physical Science Research Council
30/09/13 → 31/05/19
Project: Research Councils