Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine

Kamlesh Patel; Marcos Fernandez-villamarin; Craig Ward; Janet M. Lord; Peter Tino; Paula M. Mendes

doi:10.1039/D2AN00107A

Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine

Research output: Contribution to journal › Article › peer-review

84 Downloads (Pure)

Abstract

The kynurenine metabolite is associated with many diseases and disorders, ranging from diabetes and sepsis to more recently COVID-19. Here we report a fluorescence-based assay for the detection of kynurenine in urine using a specific chemosensor, 3-formyl-4-(ethylthio)-7-(diethylamino)-coumarin. The assay produces a linear response at clinically relevant ranges (1–20 μM), with a limit of detection of 0.7 μM. The average standard addition recoveries of kynurenine in synthetic urine samples are near to 100%, and the relative standard deviation values are less than 8%. The established fluorescence assay for quantitative analysis of kynurenine in urine is facile, sensitive and accurate and holds great potential for low-cost and high-throughput analysis of kynurenine in clinical laboratory settings.

Original language	English
Article number	1931
Pages (from-to)	1931-1936
Number of pages	6
Journal	The Analyst
Volume	147
Issue number	9
Early online date	30 Mar 2022
DOIs	https://doi.org/10.1039/D2AN00107A
Publication status	E-pub ahead of print - 30 Mar 2022

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1039/D2AN00107ALicence: Creative Commons: Attribution (CC BY)

Patel2022_Establishing__quantitative_fluorescenceFinal published version, 1.24 MBLicence: Creative Commons: Attribution (CC BY)

http://xlink.rsc.org/?DOI=D2AN00107A

FP7_ERC - GLYCOSURF
Mendes, P. (Principal Investigator)
European Commission - Management Costs, European Commission
1/12/14 → 31/05/21
Project: Research
Surface-Based Molecular Imprinting for Glycoprotein Recognition
Fossey, J. (Co-Investigator) & Mendes, P. (Principal Investigator)
Engineering & Physical Science Research Council
30/09/13 → 31/05/19
Project: Research Councils

Cite this

@article{c5b5eb1b49bd48a2ac9a547cf7ec4216,

title = "Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine",

abstract = "The kynurenine metabolite is associated with many diseases and disorders, ranging from diabetes and sepsis to more recently COVID-19. Here we report a fluorescence-based assay for the detection of kynurenine in urine using a specific chemosensor, 3-formyl-4-(ethylthio)-7-(diethylamino)-coumarin. The assay produces a linear response at clinically relevant ranges (1–20 μM), with a limit of detection of 0.7 μM. The average standard addition recoveries of kynurenine in synthetic urine samples are near to 100\%, and the relative standard deviation values are less than 8\%. The established fluorescence assay for quantitative analysis of kynurenine in urine is facile, sensitive and accurate and holds great potential for low-cost and high-throughput analysis of kynurenine in clinical laboratory settings.",

author = "Kamlesh Patel and Marcos Fernandez-villamarin and Craig Ward and Lord, \{Janet M.\} and Peter Tino and Mendes, \{Paula M.\}",

year = "2022",

month = mar,

day = "30",

doi = "10.1039/D2AN00107A",

language = "English",

volume = "147",

pages = "1931--1936",

journal = "The Analyst",

issn = "0003-2654",

publisher = "Royal Society of Chemistry",

number = "9",

}

TY - JOUR

T1 - Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine

AU - Patel, Kamlesh

AU - Fernandez-villamarin, Marcos

AU - Ward, Craig

AU - Lord, Janet M.

AU - Tino, Peter

AU - Mendes, Paula M.

PY - 2022/3/30

Y1 - 2022/3/30

N2 - The kynurenine metabolite is associated with many diseases and disorders, ranging from diabetes and sepsis to more recently COVID-19. Here we report a fluorescence-based assay for the detection of kynurenine in urine using a specific chemosensor, 3-formyl-4-(ethylthio)-7-(diethylamino)-coumarin. The assay produces a linear response at clinically relevant ranges (1–20 μM), with a limit of detection of 0.7 μM. The average standard addition recoveries of kynurenine in synthetic urine samples are near to 100%, and the relative standard deviation values are less than 8%. The established fluorescence assay for quantitative analysis of kynurenine in urine is facile, sensitive and accurate and holds great potential for low-cost and high-throughput analysis of kynurenine in clinical laboratory settings.

AB - The kynurenine metabolite is associated with many diseases and disorders, ranging from diabetes and sepsis to more recently COVID-19. Here we report a fluorescence-based assay for the detection of kynurenine in urine using a specific chemosensor, 3-formyl-4-(ethylthio)-7-(diethylamino)-coumarin. The assay produces a linear response at clinically relevant ranges (1–20 μM), with a limit of detection of 0.7 μM. The average standard addition recoveries of kynurenine in synthetic urine samples are near to 100%, and the relative standard deviation values are less than 8%. The established fluorescence assay for quantitative analysis of kynurenine in urine is facile, sensitive and accurate and holds great potential for low-cost and high-throughput analysis of kynurenine in clinical laboratory settings.

UR - https://www.scopus.com/pages/publications/85129188105

U2 - 10.1039/D2AN00107A

DO - 10.1039/D2AN00107A

M3 - Article

C2 - 35388832

SN - 0003-2654

VL - 147

SP - 1931

EP - 1936

JO - The Analyst

JF - The Analyst

IS - 9

M1 - 1931

ER -

Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine

Abstract

UN SDGs

Access to Document

Fingerprint

Projects

FP7_ERC - GLYCOSURF

Surface-Based Molecular Imprinting for Glycoprotein Recognition

Cite this