Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine

Kamlesh Patel; Marcos Fernandez-villamarin; Craig Ward; Janet M. Lord; Peter Tino; Paula M. Mendes

doi:10.1039/D2AN00107A

Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine

Kamlesh Patel, Marcos Fernandez-villamarin, Craig Ward, Janet M. Lord, Peter Tino, Paula M. Mendes

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Abstract

The kynurenine metabolite is associated with many diseases and disorders, ranging from diabetes and sepsis to more recently COVID-19. Here we report a fluorescence-based assay for the detection of kynurenine in urine using a specific chemosensor, 3-formyl-4-(ethylthio)-7-(diethylamino)-coumarin. The assay produces a linear response at clinically relevant ranges (1–20 μM), with a limit of detection of 0.7 μM. The average standard addition recoveries of kynurenine in synthetic urine samples are near to 100%, and the relative standard deviation values are less than 8%. The established fluorescence assay for quantitative analysis of kynurenine in urine is facile, sensitive and accurate and holds great potential for low-cost and high-throughput analysis of kynurenine in clinical laboratory settings.

Original language	English
Article number	1931
Pages (from-to)	1931-1936
Number of pages	6
Journal	The Analyst
Volume	147
Issue number	9
Early online date	30 Mar 2022
DOIs	https://doi.org/10.1039/D2AN00107A
Publication status	E-pub ahead of print - 30 Mar 2022

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FP7_ERC - GLYCOSURF
Mendes, P. (Principal Investigator)
European Commission, European Commission - Management Costs
1/12/14 → 31/05/21
Project: Research
Surface-Based Molecular Imprinting for Glycoprotein Recognition
Fossey, J. (Co-Investigator) & Mendes, P. (Principal Investigator)
Engineering & Physical Science Research Council
30/09/13 → 31/05/19
Project: Research Councils

Cite this

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AU - Fernandez-villamarin, Marcos

AU - Ward, Craig

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AU - Tino, Peter

AU - Mendes, Paula M.

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Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine

Abstract

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Projects

FP7_ERC - GLYCOSURF

Surface-Based Molecular Imprinting for Glycoprotein Recognition

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