Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine

Research output: Contribution to journalArticlepeer-review

Abstract

The kynurenine metabolite is associated with many diseases and disorders, ranging from diabetes and sepsis to more recently COVID-19. Here we report a fluorescence-based assay for the detection of kynurenine in urine using a specific chemosensor, 3-formyl-4-(ethylthio)-7-(diethylamino)-coumarin. The assay produces a linear response at clinically relevant ranges (1–20 μM), with a limit of detection of 0.7 μM. The average standard addition recoveries of kynurenine in synthetic urine samples are near to 100%, and the relative standard deviation values are less than 8%. The established fluorescence assay for quantitative analysis of kynurenine in urine is facile, sensitive and accurate and holds great potential for low-cost and high-throughput analysis of kynurenine in clinical laboratory settings.
Original languageEnglish
Article number1931
Pages (from-to)1931-1936
Number of pages6
JournalThe Analyst
Volume147
Issue number9
Early online date30 Mar 2022
DOIs
Publication statusE-pub ahead of print - 30 Mar 2022

Fingerprint

Dive into the research topics of 'Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine'. Together they form a unique fingerprint.

Cite this