Escherichia coli Cytochrome c Nitrite Reductase NrfA

TA Clarke; PC Mills; SR Poock; JN Butt; MR Cheesman; Jeffrey Cole; JCD Hinton; AM Hemmings; G Kemp; CAG Soderberg; S Spiro; J Van Wonderen; DJ Richardson; RK Poole

doi:10.1016/S0076-6879(07)37004-3

Escherichia coli Cytochrome c Nitrite Reductase NrfA

TA Clarke
, PC Mills
, SR Poock
, JN Butt
, MR Cheesman
, Jeffrey Cole
, JCD Hinton
, AM Hemmings
, G Kemp
, CAG Soderberg
, S Spiro
, J Van Wonderen
, DJ Richardson
, RK Poole

Biosciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

28 Citations (Scopus)

Abstract

The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.

Original language	English
Title of host publication	Methods in Enzymology
Volume	437
DOIs	https://doi.org/10.1016/S0076-6879(07)37004-3
Publication status	Published - 1 Jan 2008

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10.1016/S0076-6879(07)37004-3

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abstract = "The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.",

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AU - Clarke, TA

AU - Mills, PC

AU - Poock, SR

AU - Butt, JN

AU - Cheesman, MR

AU - Cole, Jeffrey

AU - Hinton, JCD

AU - Hemmings, AM

AU - Kemp, G

AU - Soderberg, CAG

AU - Spiro, S

AU - Van Wonderen, J

AU - Richardson, DJ

AU - Poole, RK

PY - 2008/1/1

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N2 - The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.

AB - The periplasmic cytochrome c nitrite reductase (Nrf) system of Escherichia coli utilizes nitrite as a respiratory electron acceptor by reducing it to ammonium. Nitric oxide (NO) is a proposed intermediate in this six-electron reduction and NrfA can use exogenous NO as a substrate. This chapter describes the method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.

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DO - 10.1016/S0076-6879(07)37004-3

M3 - Chapter

C2 - 18433623

SN - 9780123742780

VL - 437

BT - Methods in Enzymology

ER -

Escherichia coli Cytochrome c Nitrite Reductase NrfA

Abstract

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