Epigenetic regulation of endothelial cell lineages during zebrafish development—new insights from technical advances

Virginia Panara, Rui Monteiro, Katarzyna Koltowska*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

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Epigenetic regulation is integral in orchestrating the spatiotemporal regulation of gene expression which underlies tissue development. The emergence of new tools to assess genome-wide epigenetic modifications has enabled significant advances in the field of vascular biology in zebrafish. Zebrafish represents a powerful model to investigate the activity of cis-regulatory elements in vivo by combining technologies such as ATAC-seq, ChIP-seq and CUT&Tag with the generation of transgenic lines and live imaging to validate the activity of these regulatory elements. Recently, this approach led to the identification and characterization of key enhancers of important vascular genes, such as gata2a, notch1b and dll4. In this review we will discuss how the latest technologies in epigenetics are being used in the zebrafish to determine chromatin states and assess the function of the cis-regulatory sequences that shape the zebrafish vascular network.

Original languageEnglish
Article number891538
Pages (from-to)891538
Number of pages16
JournalFrontiers in cell and developmental biology
Publication statusPublished - 9 May 2022

Bibliographical note

Funding Information:
KK was supported by the Wallenberg Academy Fellowship (2017.0144), Ragnar Söderbergs Fellowship (M13/17), Vetenskapsådet (VR-MH-2016-01437) and Jeanssons Foundation. RM was supported by a Birmingham Fellowship (University of Birmingham, United Kingdom).

Publisher Copyright:
Copyright © 2022 Panara, Monteiro and Koltowska.


  • ATAC-seq
  • blood endothelial cells
  • ChIP-seq
  • chromatin structure
  • conserved non-coding elements
  • enhancers
  • epigenetics
  • lymphatic endothelial cells

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology


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