TY - JOUR
T1 - Enteropathogenic Escherichia coli interaction with human intestinal mucosa: role of effector proteins in brush border remodelling and 'attaching and effacing' lesion formation
AU - Shaw, Robert
AU - Cleary, Jennifer
AU - Murphy, Michael
AU - Frankel, G
AU - Knutton, Stuart
PY - 2005/1/1
Y1 - 2005/1/1
N2 - Enteropathogenic Escherichia coli (EPEC) strains deliver effector proteins Tir. EspB. Map, EspF, EspH. and EspG into host cells to induce brush border remodeling and produce attaching and effacing (A/E) lesions on small intestinal enterocytes. In this study, the role of individual EPEC effectors in brush border remodeling and A/E lesion formation was investigated with an in Nitro human small intestinal organ culture model of EPEC infection and specific effector mutants. tir, map, espB, and espH mutants produced "footprint" phenotypes due to close bacterial adhesion but subsequent loss of bacteria; an espB mutant and other type III secretion system mutants induced a "noneffacing footprint" associated with intact brush border microvilli. whereas a fir mutant was able to efface microvilli resulting in an "effacing footprint": map and espH mutants produced A/E lesions, but loss of bacteria resulted in a "pedestal footprint." An espF mutant produced typical A/E lesions without associated microvillous elongation. An espG mutant was indistinguishable from the wild type. These observations indicate that Tir, Map, EspF, and EspH effectors play a role in brush border remodeling and production of mature A/E lesions.
AB - Enteropathogenic Escherichia coli (EPEC) strains deliver effector proteins Tir. EspB. Map, EspF, EspH. and EspG into host cells to induce brush border remodeling and produce attaching and effacing (A/E) lesions on small intestinal enterocytes. In this study, the role of individual EPEC effectors in brush border remodeling and A/E lesion formation was investigated with an in Nitro human small intestinal organ culture model of EPEC infection and specific effector mutants. tir, map, espB, and espH mutants produced "footprint" phenotypes due to close bacterial adhesion but subsequent loss of bacteria; an espB mutant and other type III secretion system mutants induced a "noneffacing footprint" associated with intact brush border microvilli. whereas a fir mutant was able to efface microvilli resulting in an "effacing footprint": map and espH mutants produced A/E lesions, but loss of bacteria resulted in a "pedestal footprint." An espF mutant produced typical A/E lesions without associated microvillous elongation. An espG mutant was indistinguishable from the wild type. These observations indicate that Tir, Map, EspF, and EspH effectors play a role in brush border remodeling and production of mature A/E lesions.
UR - http://www.scopus.com/inward/record.url?scp=12844281912&partnerID=8YFLogxK
U2 - 10.1128/IAI.73.2.1243-1251.2005
DO - 10.1128/IAI.73.2.1243-1251.2005
M3 - Article
C2 - 15664974
SN - 0019-9567
VL - 73
SP - 1243
EP - 1251
JO - Infection and Immunity
JF - Infection and Immunity
ER -