Enhancement of the apparent solubility and bioavailability of Tadalafil nanoparticles via antisolvent precipitation

Qiuhong Rao, Zhenwen Qiu, Deen Huang, Tiejun Lu, Zhenyu Jason Zhang, Dandong Luo, Piaopiao Pan, Lei Zhang, Yingyan Liu, Shixia Guan, Qingguo Li

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The ability to increase the bioavailability and dissolution of poorly soluble hydrophobic drugs has been a major challenge for pharmaceutical development. This study shows that the dissolution rate, apparent solubility and oral bioavailability of tadalafil (Td) can be improved by nano-sized amorphous particles prepared by using antisolvent precipitation. Acetone and an acetone-water solution (v:v, 9:1) were selected as solvents, with deionized water as the antisolvent. The antisolvent precipitation process was conducted at a constant drug concentration of 10 mg/ml, at temperatures of 5, 10 and 15 °C and at volume ratios of antisolvent to solvent (AS/S) of 5, 8 and 10. Solid dispersion was achieved by dissolving the polymer in the antisolvent prior to the precipitation and by spray drying the suspension after the antisolvent precipitation process. The selected polymers were HPMC, VA64, and PVPK30 at concentrations of 33, 100 and 300 mg per 100 ml of water (equivalent to weight ratios of drug-to-polymer of 1:3, 1:1 and 3:1, respectively). The solid dispersions were characterized by scanning electron microscopy (SEM), X-ray powder diffraction (XRPD), differential scanning calorimetry (DSC) and fourier transform infrared spectroscopy (FT-IR). The improvements in the dissolution rate, equilibrium solubility, apparent solubility and bioavailability were tested and compared with unprocessed Td. Td particles in the suspension (before spray drying) were 200 nm, and the obtained Td solid dispersion had a size of approximately 5–10 μm. The XRPD, DSC and FT-IR analyses confirmed that the prepared Td particles in the solid dispersions were amorphous. The solid dispersion obtained using the optimized process conditions exhibited 8.5 times faster dissolution rates in the first minute of dissolution, 22 times greater apparent solubility at 10 min and a 3.67-fold increase in oral bioavailability than the as-received Td. The present work demonstrated that low temperature antisolvent precipitation technique has excellent potential to prepare nano-sized amorphous particles with a faster release and a higher bioavailability.

Original languageEnglish
Pages (from-to)222-231
Number of pages10
JournalEuropean Journal of Pharmaceutical Sciences
Volume128
DOIs
Publication statusPublished - 1 Feb 2019

Bibliographical note

Funding Information:
The authors acknowledge financial support from the Science Program for Overseas Scholars of Guangzhou University of Chinese Medicine (Torch program), the Guangdong Science and Technology Program (2017ZC0140; 2017ZC0157), the School of Chemical Engineering, University of Birmingham, and the School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine.

Funding Information:
The authors acknowledge financial support from the Science Program for Overseas Scholars of Guangzhou University of Chinese Medicine (Torch program), the Guangdong Science and Technology Program ( 2017ZC0140 ; 2017ZC0157 ), the School of Chemical Engineering, University of Birmingham , and the School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine .

Publisher Copyright:
© 2018

Keywords

  • Amorphous solid dispersion
  • Antisolvent precipitation
  • Apparent solubility
  • Bioavailability
  • Tadalafil

ASJC Scopus subject areas

  • Pharmaceutical Science

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