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Abstract
Klebsiella pneumoniae strains carrying OXA-48-like carbapenemases are increasingly prevalent across the globe. There is thus an urgent need to better understand the mechanisms that underpin the dissemination of bla OXA-48-like carbapenemases. To this end, four ertapenem-resistant K. pneumoniae isolates producing OXA-48-like carbapenemases were isolated from two patients. Genome sequencing revealed that one sequence type (ST) 17 isolate carried bla OXA-181, whilst three isolates from a single patient, two ST76 and one ST15, carried bla OXA-232. The 50514 bp bla OXA-181-harbouring plasmid, pOXA-181_YML0508, was X3-type with a conjugation frequency to Escherichia coli of 1.94×10-4 transconjugants per donor. The bla OXA-232 gene was located on a 6141 bp ColKP3-type plasmid, pOXA-232_WSD, that was identical in the ST76 and ST15 K. pneumoniae isolates. This plasmid could be transferred from K. pneumoniae to E. coli at low frequency, 8.13×10-6 transconjugants per donor. Comparative analysis revealed that the X3 plasmid acquired the bla OXA-48-like gene via IS3000-mediated co-integration of the ColKP3-type plasmid. Our study highlights how plasmid integration and rearrangements can contribute to the spread of bla OXA-48-like genes, which provides important clues for clinical prevention of the dissemination of K. pneumoniae strains carrying bla OXA-48-like carbapenemases.
Original language | English |
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Article number | 001306 |
Journal | Journal of Medical Microbiology |
Volume | 70 |
Issue number | 3 |
Early online date | 28 Jan 2021 |
DOIs | |
Publication status | Published - Mar 2021 |
Keywords
- Aged, 80 and over
- Bacterial Proteins/genetics
- Carbapenem-Resistant Enterobacteriaceae/genetics
- China
- Genes, Bacterial
- Humans
- Klebsiella Infections/microbiology
- Klebsiella pneumoniae/drug effects
- Male
- Middle Aged
- Plasmids
- beta-Lactamases/genetics
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- 1 Finished
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DETECTIVE: Dissemination and resistance mechanisms of carbapenem-resistant Gram-negative bacilli
14/02/19 → 31/03/23
Project: Research Councils