Wound care management is a serious issue among the medical practitioners due to its varying complexity and various materials were tested for fast relief and easy removal. In this regard zwitterionic polymer based wound dressing membranes are the key point of attraction. Here we prepared a novel zwitterionic poly (carboxybetaine-co-methyl methacrylate) (CBMA) copolymer based nanomembranes using the electrospinning technique for the wound dressing application. The study takes advantage of the outstanding chemical properties of zwitterionic CBMA and the morphological efficiency of nanomembranes. The cell attachment studies proved the cell inert nature of thus prepared membranes. Such non cell adherent wound dressing membranes can be applied as the easy removable, no-pain wound dressing bandages. Our results clearly showed that the excellent blood-inert nature can be achieved by the CBMA nanofiber membranes. Therefore, there will be less chance of attaching blood clot with the wound dressing membrane and is extremely significant for the care of patients with large areas of chronic wounds. Additionally the in vivo results showed the formation of new tissues within two weeks, evidence of a complete wound healing material. So our CBMA membrane can be successfully used as a perfect wound dressing material with minimum cosmetic scar.