Efflux in Acinetobacter baumannii can be determined by measuring accumulation of H33342 (bis-benzamide)

G E Richmond, K L Chua, L J V Piddock

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Overexpression of efflux pumps in Acinetobacter baumannii is a common mechanism of multidrug resistance in this nosocomial pathogen. Increased efflux pump expression is often assumed from MICs of antibiotics and dyes, without measurement of efflux levels. This study describes a safe, rapid and simple 96-well plate assay that measures the accumulation of a fluorescent dye, Hoechst (H) 33342.
Original languageEnglish
Pages (from-to)1594-600
Number of pages7
JournalJournal of Antimicrobial Chemotherapy
Volume68
Issue number7
DOIs
Publication statusPublished - Jul 2013

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