Abstract
Overexpression of efflux pumps in Acinetobacter baumannii is a common mechanism of multidrug resistance in this nosocomial pathogen. Increased efflux pump expression is often assumed from MICs of antibiotics and dyes, without measurement of efflux levels. This study describes a safe, rapid and simple 96-well plate assay that measures the accumulation of a fluorescent dye, Hoechst (H) 33342.
Original language | English |
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Pages (from-to) | 1594-600 |
Number of pages | 7 |
Journal | Journal of Antimicrobial Chemotherapy |
Volume | 68 |
Issue number | 7 |
DOIs | |
Publication status | Published - Jul 2013 |