Effects of Morphogen and Scaffold Porogen on the Differentiation of Dental Pulp Stem Cells

FF Demarco, L Casagrande, Z Zhang, Z Dong, SB Tarquinio, BD Zeitlin, S Shi, Anthony Smith, JE Nör

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

INTRODUCTION: Dental pulp tissue engineering is an emerging field that can potentially have a major impact on oral health. However, the source of morphogens required for stem cell differentiation into odontoblasts and the scaffold characteristics that are more conducive to odontoblastic differentiation are still unclear. This study investigated the effect of dentin and scaffold porogen on the differentiation of human dental pulp stem cells (DPSCs) into odontoblasts. METHODS: Poly-L-lactic acid (PLLA) scaffolds were prepared in pulp chambers of extracted human third molars using salt crystals or gelatin spheres as porogen. DPSCs seeded in tooth slice/scaffolds or control scaffolds (without tooth slice) were either cultured in vitro or implanted subcutaneously in immunodefficient mice. RESULTS: DPSCs seeded in tooth slice/scaffolds but not in control scaffolds expressed putative odontoblastic markers (DMP-1, DSPP, and MEPE) in vitro and in vivo. DPSCs seeded in tooth/slice scaffolds presented lower proliferation rates than in control scaffolds between 7 and 21 days (p <0.05). DPSCs seeded in tooth slice/scaffolds and transplanted into mice generated a tissue with morphological characteristics similar to those of human dental pulps. Scaffolds generated with gelatin or salt porogen resulted in similar DPSC proliferation. The porogen type had a relatively modest impact on the expression of the markers of odontoblastic differentiation. CONCLUSIONS: Collectively, this work shows that dentin-related morphogens are important for the differentiation of DPSC into odontoblasts and for the engineering of dental pulp-like tissues and suggest that environmental cues influence DPSC behavior and differentiation potential.
Original languageEnglish
Pages (from-to)1805-1811
Number of pages7
JournalJournal of Endodontics
Volume36
Issue number11
Early online date20 Sep 2010
DOIs
Publication statusPublished - 1 Nov 2010

Keywords

  • tissue engineering
  • Dentin
  • odontoblast
  • pulp regeneration
  • endodontics

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