Effects of long-term exposure of gelatinated and non-gelatinated cadmium telluride quantum dots on differentiated PC12 cells

Babu R. Prasad; Gillian Mullins; Natalia Nikolskaya; David Connolly; Terry J. Smith; Valérie A. Gérard; Stephen J. Byrne; Gemma Louise Davies; Yurii K. Gun'ko; Yury Rochev

doi:10.1186/1477-3155-10-4

Effects of long-term exposure of gelatinated and non-gelatinated cadmium telluride quantum dots on differentiated PC12 cells

Babu R. Prasad, Gillian Mullins, Natalia Nikolskaya, David Connolly, Terry J. Smith, Valérie A. Gérard, Stephen J. Byrne, Gemma Louise Davies, Yurii K. Gun'ko, Yury Rochev^*

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Background: The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy.Results: Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs.Conclusion: The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days).

Original language	English
Article number	4
Journal	Journal of Nanobiotechnology
Volume	10
DOIs	https://doi.org/10.1186/1477-3155-10-4
Publication status	Published - 20 Jan 2012

Bibliographical note

Funding Information:
The authors would like to thank Dr. Ayswaria Deepti, Apoptosis Group, NCBES, National University of Ireland, Galway and Dr. Kerry Thomson, Department of Anatomy, School of Medicine, National University of Ireland, Galway, Ireland. This work was funded by Science Foundation Ireland (SFI).

Keywords

Apoptosis
CdTe quantum dots
Cytotoxicity
Differentiated PC12 cells
Neuronal growth factor

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Molecular Medicine
Biomedical Engineering
Applied Microbiology and Biotechnology
Pharmaceutical Science

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10.1186/1477-3155-10-4

Cite this

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title = "Effects of long-term exposure of gelatinated and non-gelatinated cadmium telluride quantum dots on differentiated PC12 cells",

abstract = "Background: The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy.Results: Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs.Conclusion: The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days).",

keywords = "Apoptosis, CdTe quantum dots, Cytotoxicity, Differentiated PC12 cells, Neuronal growth factor",

author = "Prasad, {Babu R.} and Gillian Mullins and Natalia Nikolskaya and David Connolly and Smith, {Terry J.} and G{\'e}rard, {Val{\'e}rie A.} and Byrne, {Stephen J.} and Davies, {Gemma Louise} and Gun'ko, {Yurii K.} and Yury Rochev",

note = "Funding Information: The authors would like to thank Dr. Ayswaria Deepti, Apoptosis Group, NCBES, National University of Ireland, Galway and Dr. Kerry Thomson, Department of Anatomy, School of Medicine, National University of Ireland, Galway, Ireland. This work was funded by Science Foundation Ireland (SFI).",

year = "2012",

month = jan,

day = "20",

doi = "10.1186/1477-3155-10-4",

language = "English",

volume = "10",

journal = "Journal of Nanobiotechnology",

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T1 - Effects of long-term exposure of gelatinated and non-gelatinated cadmium telluride quantum dots on differentiated PC12 cells

AU - Prasad, Babu R.

AU - Mullins, Gillian

AU - Nikolskaya, Natalia

AU - Connolly, David

AU - Smith, Terry J.

AU - Gérard, Valérie A.

AU - Byrne, Stephen J.

AU - Davies, Gemma Louise

AU - Gun'ko, Yurii K.

AU - Rochev, Yury

N1 - Funding Information: The authors would like to thank Dr. Ayswaria Deepti, Apoptosis Group, NCBES, National University of Ireland, Galway and Dr. Kerry Thomson, Department of Anatomy, School of Medicine, National University of Ireland, Galway, Ireland. This work was funded by Science Foundation Ireland (SFI).

PY - 2012/1/20

Y1 - 2012/1/20

N2 - Background: The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy.Results: Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs.Conclusion: The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days).

AB - Background: The inherent toxicity of unmodified Quantum Dots (QDs) is a major hindrance to their use in biological applications. To make them more potent as neuroprosthetic and neurotherapeutic agents, thioglycolic acid (TGA) capped CdTe QDs, were coated with a gelatine layer and investigated in this study with differentiated pheochromocytoma 12 (PC12) cells. The QD - cell interactions were investigated after incubation periods of up to 17 days by MTT and APOTOX-Glo Triplex assays along with using confocal microscopy.Results: Long term exposure (up to 17 days) to gelatinated TGA-capped CdTe QDs of PC12 cells in the course of differentiation and after neurites were grown resulted in dramatically reduced cytotoxicity compared to non-gelatinated TGA-capped CdTe QDs.Conclusion: The toxicity mechanism of QDs was identified as caspase-mediated apoptosis as a result of cadmium leaking from the core of QDs. It was therefore concluded that the gelatine capping on the surface of QDs acts as a barrier towards the leaking of toxic ions from the core QDs in the long term (up to 17 days).

KW - Apoptosis

KW - CdTe quantum dots

KW - Cytotoxicity

KW - Differentiated PC12 cells

KW - Neuronal growth factor

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Effects of long-term exposure of gelatinated and non-gelatinated cadmium telluride quantum dots on differentiated PC12 cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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