Effects of alginate hydrogels and TGF-beta 1 on human dental pulp repair in vitro.

K Dobie, G Smith, Alastair Sloan, Anthony Smith

Research output: Contribution to journalArticle

101 Citations (Scopus)


The objective of this study was to investigate the use of alginate hydrogels to present either exogenous or endogenous transforming growth factor (TGF)-beta 1 to the dentin-pulp complex to signal reparative processes. Hydrogels were prepared, applied to cultured human tooth slices and the effects on tertiary dentinogenesis examined histologically. Both TGF-beta 1-containing and acid-treated alginate hydrogels, but not untreated hydrogels, upregulated dentin matrix secretion and induced odontoblast-like cell differentiation with subsequent secretion of regular tubular dentin matrix on cut pulpal surfaces. It is concluded that TGF-beta 1 can signal both induction of odontoblast-like cell differentiation and upregulation of their matrix secretion in the human dentin-pulp complex. Alginate hydrogels provide an appropriate matrix in which dental regeneration can take place and may also be useful for delivery of growth factors, including TGF-beta s, to enhance the natural regenerative capacity of the dental pulp.
Original languageEnglish
Pages (from-to)387-90
Number of pages4
JournalConnective Tissue Research
Issue number2-3
Publication statusPublished - 1 Jan 2002


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