Effcient enzymatic hydrolysis of biomass hemicellulose in the absence of bulkwater

Shaghayegh Ostadjoo; Fabien Hammerer; Karolin Dietrich; Marie Josée Dumont; Tomislav Friscic; Karine Auclair

doi:10.3390/molecules24234206

Effcient enzymatic hydrolysis of biomass hemicellulose in the absence of bulkwater

Shaghayegh Ostadjoo, Fabien Hammerer, Karolin Dietrich, Marie Josée Dumont, Tomislav Friscic^*, Karine Auclair

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Current enzymatic methods for hemicellulosic biomass depolymerization are solution-based, typically require a harsh chemical pre-treatment of the material and large volumes of water, yet lack in efficiency. In our study, xylanase (E.C. 3.2.1.8) from Thermomyces lanuginosus is used to hydrolyze xylans from different sources. We report an innovative enzymatic process which avoids the use of bulk aqueous, organic or inorganic solvent, and enables hydrolysis of hemicellulose directly from chemically untreated biomass, to low-weight, soluble oligoxylosaccharides in >70% yields.

Original language	English
Article number	4206
Journal	Molecules
Volume	24
Issue number	23
DOIs	https://doi.org/10.3390/molecules24234206
Publication status	Published - 20 Nov 2019

Bibliographical note

Publisher Copyright:
© 2019 by the authors.

Keywords

Biowaste
Glycosyl hydrolase
Hemicellulase
Hemicellulose
Mechanochemistry
Mechanoenzymology
Saccharification
Solvent-free
Xylan
Xylanase

ASJC Scopus subject areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

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10.3390/molecules24234206

Cite this

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title = "Effcient enzymatic hydrolysis of biomass hemicellulose in the absence of bulkwater",

abstract = "Current enzymatic methods for hemicellulosic biomass depolymerization are solution-based, typically require a harsh chemical pre-treatment of the material and large volumes of water, yet lack in efficiency. In our study, xylanase (E.C. 3.2.1.8) from Thermomyces lanuginosus is used to hydrolyze xylans from different sources. We report an innovative enzymatic process which avoids the use of bulk aqueous, organic or inorganic solvent, and enables hydrolysis of hemicellulose directly from chemically untreated biomass, to low-weight, soluble oligoxylosaccharides in >70\% yields.",

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AU - Ostadjoo, Shaghayegh

AU - Hammerer, Fabien

AU - Dietrich, Karolin

AU - Dumont, Marie Josée

AU - Friscic, Tomislav

AU - Auclair, Karine

PY - 2019/11/20

Y1 - 2019/11/20

N2 - Current enzymatic methods for hemicellulosic biomass depolymerization are solution-based, typically require a harsh chemical pre-treatment of the material and large volumes of water, yet lack in efficiency. In our study, xylanase (E.C. 3.2.1.8) from Thermomyces lanuginosus is used to hydrolyze xylans from different sources. We report an innovative enzymatic process which avoids the use of bulk aqueous, organic or inorganic solvent, and enables hydrolysis of hemicellulose directly from chemically untreated biomass, to low-weight, soluble oligoxylosaccharides in >70% yields.

AB - Current enzymatic methods for hemicellulosic biomass depolymerization are solution-based, typically require a harsh chemical pre-treatment of the material and large volumes of water, yet lack in efficiency. In our study, xylanase (E.C. 3.2.1.8) from Thermomyces lanuginosus is used to hydrolyze xylans from different sources. We report an innovative enzymatic process which avoids the use of bulk aqueous, organic or inorganic solvent, and enables hydrolysis of hemicellulose directly from chemically untreated biomass, to low-weight, soluble oligoxylosaccharides in >70% yields.

KW - Biowaste

KW - Glycosyl hydrolase

KW - Hemicellulase

KW - Hemicellulose

KW - Mechanochemistry

KW - Mechanoenzymology

KW - Saccharification

KW - Solvent-free

KW - Xylan

KW - Xylanase

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U2 - 10.3390/molecules24234206

DO - 10.3390/molecules24234206

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SN - 1420-3049

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JO - Molecules

JF - Molecules

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ER -

Effcient enzymatic hydrolysis of biomass hemicellulose in the absence of bulkwater

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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