EBV-specific CD4+ T cell clones exhibit vigorous allogeneic responses

E Landais, A Morice, Heather Long, Tracey Haigh, B Charreau, M Bonneville, Graham Taylor, E Houssaint

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Alloreactive T cells play a key role in mediating graft-vs-host disease and allograft rejection, and recent data suggest that most T cell alloreactivity resides within the CD4 T cell subset. Particularly, T cell responses to herpesvirus can shape the alloreactive repertoire and influence transplantation outcomes. In this study, we describe six distinct EBV-specific CD4(+) T cell clones that cross-reacted with EBV-transformed lymphoblastoid cell lines (LCLs), dendritic cells, and endothelial cells expressing MHC class H alleles commonly found in the population. Allorecognition showed exquisite MHC specificity. These CD4(+) T cell clones efficiently killed dendritic cells or LCLs expressing the cross-reactive allogeneic MHC class H molecules, whereas they did not kill autologous LCLs. Endothelial cells expressing the proper allogeneic MHC molecules were poorly killed, but they induced high-level TNF-alpha production by the EBV-specific CD4(+) T cell clones. As already proposed, the strong alloreactivity toward LCLs suggest that these cells could be used for selective depletion of alloreactive T cells.
Original languageEnglish
Pages (from-to)1427-1433
Number of pages7
JournalJournal of Immunology
Volume177
Issue number3
Publication statusPublished - 1 Aug 2006

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