Dual RNASeq unveils NTHi-macrophage transcriptomic changes during intracellular persistence

Jodie Ackland; Ashley Heinson; David Cleary; Myron Christodoulides; Tom Wilkinson; Karl Staples

doi:10.1183/13993003.congress-2020.2323

Dual RNASeq unveils NTHi-macrophage transcriptomic changes during intracellular persistence

Jodie Ackland
, Ashley Heinson
, David Cleary
, Myron Christodoulides
, Tom Wilkinson
, Karl Staples

Microbes, Infection and Microbiomes

Research output: Contribution to journal › Article › peer-review

Abstract

Nontypeable Haemophilus influenzae (NTHi) is a respiratory tract pathogen associated with severe, neutrophilic asthma. Although macrophages are responsible for orchestrating the immune response and pathogen clearance in the lung, NTHi is able to persist within macrophages. The mechanism of NTHi intracellular persistence is not understood, therefore the aim of this work was to use dual RNASeq to investigate the host-pathogen interactions that allow this persistence. Monocyte-derived macrophages (MDM) were used to model NTHi-macrophage infection. RNA was isolated after 6h and 24h of infection and sequenced using the Illumina NovaSeq 6000 platform. Differential gene expression analysis found expression of 863 MDM genes (FDR p

Original language	English
Journal	European Respiratory Journal
Volume	56
Issue number	Suppl. 64
DOIs	https://doi.org/10.1183/13993003.congress-2020.2323
Publication status	Published - 28 Oct 2020

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title = "Dual RNASeq unveils NTHi-macrophage transcriptomic changes during intracellular persistence",

abstract = "Nontypeable Haemophilus influenzae (NTHi) is a respiratory tract pathogen associated with severe, neutrophilic asthma. Although macrophages are responsible for orchestrating the immune response and pathogen clearance in the lung, NTHi is able to persist within macrophages. The mechanism of NTHi intracellular persistence is not understood, therefore the aim of this work was to use dual RNASeq to investigate the host-pathogen interactions that allow this persistence. Monocyte-derived macrophages (MDM) were used to model NTHi-macrophage infection. RNA was isolated after 6h and 24h of infection and sequenced using the Illumina NovaSeq 6000 platform. Differential gene expression analysis found expression of 863 MDM genes (FDR p",

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AU - Ackland, Jodie

AU - Heinson, Ashley

AU - Cleary, David

AU - Christodoulides, Myron

AU - Wilkinson, Tom

AU - Staples, Karl

PY - 2020/10/28

Y1 - 2020/10/28

N2 - Nontypeable Haemophilus influenzae (NTHi) is a respiratory tract pathogen associated with severe, neutrophilic asthma. Although macrophages are responsible for orchestrating the immune response and pathogen clearance in the lung, NTHi is able to persist within macrophages. The mechanism of NTHi intracellular persistence is not understood, therefore the aim of this work was to use dual RNASeq to investigate the host-pathogen interactions that allow this persistence. Monocyte-derived macrophages (MDM) were used to model NTHi-macrophage infection. RNA was isolated after 6h and 24h of infection and sequenced using the Illumina NovaSeq 6000 platform. Differential gene expression analysis found expression of 863 MDM genes (FDR p

AB - Nontypeable Haemophilus influenzae (NTHi) is a respiratory tract pathogen associated with severe, neutrophilic asthma. Although macrophages are responsible for orchestrating the immune response and pathogen clearance in the lung, NTHi is able to persist within macrophages. The mechanism of NTHi intracellular persistence is not understood, therefore the aim of this work was to use dual RNASeq to investigate the host-pathogen interactions that allow this persistence. Monocyte-derived macrophages (MDM) were used to model NTHi-macrophage infection. RNA was isolated after 6h and 24h of infection and sequenced using the Illumina NovaSeq 6000 platform. Differential gene expression analysis found expression of 863 MDM genes (FDR p

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DO - 10.1183/13993003.congress-2020.2323

M3 - Article

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VL - 56

JO - European Respiratory Journal

JF - European Respiratory Journal

IS - Suppl. 64

ER -

Dual RNASeq unveils NTHi-macrophage transcriptomic changes during intracellular persistence

Abstract

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