Down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation

Francesca Lessi; Andrew Beggs; Mariagrazia de Palo; Marcello Anti; Raffaele Macarone Palmieri; Simona Francesconi; Vito Gomes; Generoso Bevilacqua; Ian Tomlinson; Stefania Segditsas

doi:10.1371/journal.pone.0013840

Down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation

Francesca Lessi, Andrew Beggs, Mariagrazia de Palo, Marcello Anti, Raffaele Macarone Palmieri, Simona Francesconi, Vito Gomes, Generoso Bevilacqua, Ian Tomlinson, Stefania Segditsas

Cancer and Genomic Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We have previously shown that serum/glucocorticoid regulated kinase 1 (SGK1) is down-regulated in colorectal cancers (CRC) with respect to normal tissue. As hyper-methylation of promoter regions is a well-known mechanism of gene silencing in cancer, we tested whether the SGK1 promoter region was methylated in colonic tumour samples.

Original language	English
Pages (from-to)	e13840
Journal	PLoS ONE
Volume	5
Issue number	11
DOIs	https://doi.org/10.1371/journal.pone.0013840
Publication status	Published - 2010

Keywords

Polymorphism, Single Nucleotide
Humans
Receptors, Glucocorticoid
Cell Line, Tumor
Enzyme Inhibitors
HCT116 Cells
Reverse Transcriptase Polymerase Chain Reaction
Protein-Serine-Threonine Kinases
Gene Expression Regulation, Neoplastic
Promoter Regions, Genetic
Blotting, Western
DNA Methylation
Down-Regulation
CpG Islands
Azacitidine
Immediate-Early Proteins
HT29 Cells
Colorectal Neoplasms

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1371/journal.pone.0013840

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title = "Down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation",

abstract = "We have previously shown that serum/glucocorticoid regulated kinase 1 (SGK1) is down-regulated in colorectal cancers (CRC) with respect to normal tissue. As hyper-methylation of promoter regions is a well-known mechanism of gene silencing in cancer, we tested whether the SGK1 promoter region was methylated in colonic tumour samples.",

keywords = "Polymorphism, Single Nucleotide, Humans, Receptors, Glucocorticoid, Cell Line, Tumor, Enzyme Inhibitors, HCT116 Cells, Reverse Transcriptase Polymerase Chain Reaction, Protein-Serine-Threonine Kinases, Gene Expression Regulation, Neoplastic, Promoter Regions, Genetic, Blotting, Western, DNA Methylation, Down-Regulation, CpG Islands, Azacitidine, Immediate-Early Proteins, HT29 Cells, Colorectal Neoplasms",

author = "Francesca Lessi and Andrew Beggs and {de Palo}, Mariagrazia and Marcello Anti and {Macarone Palmieri}, Raffaele and Simona Francesconi and Vito Gomes and Generoso Bevilacqua and Ian Tomlinson and Stefania Segditsas",

year = "2010",

doi = "10.1371/journal.pone.0013840",

language = "English",

volume = "5",

pages = "e13840",

journal = "PLoS ONE",

issn = "1932-6203",

publisher = "Public Library of Science (PLOS)",

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TY - JOUR

T1 - Down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation

AU - Lessi, Francesca

AU - Beggs, Andrew

AU - de Palo, Mariagrazia

AU - Anti, Marcello

AU - Macarone Palmieri, Raffaele

AU - Francesconi, Simona

AU - Gomes, Vito

AU - Bevilacqua, Generoso

AU - Tomlinson, Ian

AU - Segditsas, Stefania

PY - 2010

Y1 - 2010

N2 - We have previously shown that serum/glucocorticoid regulated kinase 1 (SGK1) is down-regulated in colorectal cancers (CRC) with respect to normal tissue. As hyper-methylation of promoter regions is a well-known mechanism of gene silencing in cancer, we tested whether the SGK1 promoter region was methylated in colonic tumour samples.

AB - We have previously shown that serum/glucocorticoid regulated kinase 1 (SGK1) is down-regulated in colorectal cancers (CRC) with respect to normal tissue. As hyper-methylation of promoter regions is a well-known mechanism of gene silencing in cancer, we tested whether the SGK1 promoter region was methylated in colonic tumour samples.

KW - Polymorphism, Single Nucleotide

KW - Humans

KW - Receptors, Glucocorticoid

KW - Cell Line, Tumor

KW - Enzyme Inhibitors

KW - HCT116 Cells

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Protein-Serine-Threonine Kinases

KW - Gene Expression Regulation, Neoplastic

KW - Promoter Regions, Genetic

KW - Blotting, Western

KW - DNA Methylation

KW - Down-Regulation

KW - CpG Islands

KW - Azacitidine

KW - Immediate-Early Proteins

KW - HT29 Cells

KW - Colorectal Neoplasms

U2 - 10.1371/journal.pone.0013840

DO - 10.1371/journal.pone.0013840

M3 - Article

C2 - 21079778

SN - 1932-6203

VL - 5

SP - e13840

JO - PLoS ONE

JF - PLoS ONE

IS - 11

ER -

Down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation

Abstract

Keywords

UN SDGs

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