Does deamidation of islet amyloid polypeptide accelerate amyloid fibril formation?

Yuko P. Y. Lam; Christopher A. Wootton; Ian J. Hands-portman; Juan Wei; Cookson K. C. Chiu; Isolda Romero-Canelón; Frederik Lermyte; Mark P. Barrow; Peter B. O'connor

doi:10.1039/C8CC06675B

Does deamidation of islet amyloid polypeptide accelerate amyloid fibril formation?

Yuko P. Y. Lam, Christopher A. Wootton, Ian J. Hands-portman, Juan Wei, Cookson K. C. Chiu, Isolda Romero-Canelón, Frederik Lermyte, Mark P. Barrow, Peter B. O'connor

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Abstract

Mass spectrometry has been applied to determine the deamidation sites and the aggregation region of the deamidated human islet amyloid polypeptide (hIAPP). Mutant hIAPP with iso-aspartic residue mutations at possible deamidation sites showed very different fibril formation behaviour, which correlates with the observed deamidation-induced acceleration of hIAPP aggregation.

Original language	English
Pages (from-to)	13853-13856
Number of pages	4
Journal	Chemical Communications
Volume	54
Issue number	98
DOIs	https://doi.org/10.1039/C8CC06675B
Publication status	Published - 19 Nov 2018

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10.1039/C8CC06675BLicence: None: All rights reserved

Lam_et_al_Does_deamidation_of_islet_amyloid_Chemical_Communications_2018
Checked for eligibility 07/12/2018 This is a peer-reviewed version of an article published in Chemical Communications. Chem. Commun., 2018,54, 13853-13856 10.1039/C8CC06675B
Accepted author manuscript, 2.93 MBLicence: None: All rights reserved

https://pubs.rsc.org/en/content/articlelanding/2018/cc/c8cc06675bLicence: None: All rights reserved

Cite this

@article{0a679e702cf0476c868aa39c51cd4e81,

title = "Does deamidation of islet amyloid polypeptide accelerate amyloid fibril formation?",

abstract = "Mass spectrometry has been applied to determine the deamidation sites and the aggregation region of the deamidated human islet amyloid polypeptide (hIAPP). Mutant hIAPP with iso-aspartic residue mutations at possible deamidation sites showed very different fibril formation behaviour, which correlates with the observed deamidation-induced acceleration of hIAPP aggregation.",

author = "Lam, {Yuko P. Y.} and Wootton, {Christopher A.} and Hands-portman, {Ian J.} and Juan Wei and Chiu, {Cookson K. C.} and Isolda Romero-Canel{\'o}n and Frederik Lermyte and Barrow, {Mark P.} and O'connor, {Peter B.}",

year = "2018",

month = nov,

day = "19",

doi = "10.1039/C8CC06675B",

language = "English",

volume = "54",

pages = "13853--13856",

journal = "Chemical Communications ",

issn = "1359-7345",