Over the past decade, plant DNA barcoding has emerged as a scientific breakthrough and is often used to help with species identification or as a taxonomical tool. DNA barcoding is very important in medicinal plant use, not only for identification purposes but also for the authentication of medicinal products. Here, a total of 61 Indonesian medicinal plant species from 30 families and a pair of ITS2, matK, rbcL, and trnL primers were used for a DNA barcoding study consisting of molecular and sequence analyses. This study aimed to analyze how the four identified DNA barcoding regions (ITS2, matK, rbcL, and trnL) aid identification and conservation and to investigate their effectiveness for DNA barcoding for the studied species. This study resulted in 212 DNA barcoding sequences and identified new ones for the studied medicinal plant species. Though there is no ideal or perfect region for DNA barcoding of the target species, we recommend matK as the main region for Indonesian medicinal plant identification, with ITS2 and rbcL as alternative or complementary regions. These findings will be useful for forensic studies that support the conservation of medicinal plants and their national and global use.
Bibliographical noteFunding Information:
This study was funded by the Ministry of Finance of the Republic of Indonesia, grant number 20160722038259 through the Indonesia Endowment Fund for Education (LPDP) through R. Cahyaningsih’s scholarship and The APC was funded by the University of Birmingham, UK. Acknowledgments: We thank the Registration and Nursery Subdivision of Bogor Botanic Gardens (BBG) and Cibodas Botanic Gardens (CBD), Indonesian Institute of Sciences (LIPI) and Hortus Botanicus Leiden (HBL), the Netherlands for providing the samples for DNA barcoding. Most of samples are from BBG, except Amomum hochreutineri Valeton, Etlingera solaris (Blume) R.M.Sm., Psychotria montana Blume, Rhododendron macgregoriae F.Muell., Smilax calophylla Wall. ex A.DC. and Staurogyne elongate (Nees) Kuntze are from CBG, and Aglaonema commutatum Schott, Ardisia complanate Wall., Cymbidium ensifolium (L.) Sw., and Hoya diversifolia Blume are from HBL.
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- DNA barcoding
- medicinal plants