DNA barcoding medicinal plant species from Indonesia

Ria Cahyaningsih; Lindsey Jane Compton; Sri Rahayu; Joana Magos Brehm; Nigel Maxted

doi:10.3390/plants11101375

DNA barcoding medicinal plant species from Indonesia

Ria Cahyaningsih, Lindsey Jane Compton, Sri Rahayu, Joana Magos Brehm, Nigel Maxted

Biosciences

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Abstract

Over the past decade, plant DNA barcoding has emerged as a scientific breakthrough and is often used to help with species identification or as a taxonomical tool. DNA barcoding is very important in medicinal plant use, not only for identification purposes but also for the authentication of medicinal products. Here, a total of 61 Indonesian medicinal plant species from 30 families and a pair of ITS2, matK, rbcL, and trnL primers were used for a DNA barcoding study consisting of molecular and sequence analyses. This study aimed to analyze how the four identified DNA barcoding regions (ITS2, matK, rbcL, and trnL) aid identification and conservation and to investigate their effectiveness for DNA barcoding for the studied species. This study resulted in 212 DNA barcoding sequences and identified new ones for the studied medicinal plant species. Though there is no ideal or perfect region for DNA barcoding of the target species, we recommend matK as the main region for Indonesian medicinal plant identification, with ITS2 and rbcL as alternative or complementary regions. These findings will be useful for forensic studies that support the conservation of medicinal plants and their national and global use.

Original language	English
Article number	1375
Number of pages	22
Journal	Plants
Volume	11
Issue number	10
DOIs	https://doi.org/10.3390/plants11101375
Publication status	Published - 21 May 2022

Bibliographical note

Funding Information:
This study was funded by the Ministry of Finance of the Republic of Indonesia, grant number 20160722038259 through the Indonesia Endowment Fund for Education (LPDP) through R. Cahyaningsih’s scholarship and The APC was funded by the University of Birmingham, UK. Acknowledgments: We thank the Registration and Nursery Subdivision of Bogor Botanic Gardens (BBG) and Cibodas Botanic Gardens (CBD), Indonesian Institute of Sciences (LIPI) and Hortus Botanicus Leiden (HBL), the Netherlands for providing the samples for DNA barcoding. Most of samples are from BBG, except Amomum hochreutineri Valeton, Etlingera solaris (Blume) R.M.Sm., Psychotria montana Blume, Rhododendron macgregoriae F.Muell., Smilax calophylla Wall. ex A.DC. and Staurogyne elongate (Nees) Kuntze are from CBG, and Aglaonema commutatum Schott, Ardisia complanate Wall., Cymbidium ensifolium (L.) Sw., and Hoya diversifolia Blume are from HBL.

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

DNA barcoding
medicinal plants
conservation
forensic
Indonesia

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Cite this

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title = "DNA barcoding medicinal plant species from Indonesia",

abstract = "Over the past decade, plant DNA barcoding has emerged as a scientific breakthrough and is often used to help with species identification or as a taxonomical tool. DNA barcoding is very important in medicinal plant use, not only for identification purposes but also for the authentication of medicinal products. Here, a total of 61 Indonesian medicinal plant species from 30 families and a pair of ITS2, matK, rbcL, and trnL primers were used for a DNA barcoding study consisting of molecular and sequence analyses. This study aimed to analyze how the four identified DNA barcoding regions (ITS2, matK, rbcL, and trnL) aid identification and conservation and to investigate their effectiveness for DNA barcoding for the studied species. This study resulted in 212 DNA barcoding sequences and identified new ones for the studied medicinal plant species. Though there is no ideal or perfect region for DNA barcoding of the target species, we recommend matK as the main region for Indonesian medicinal plant identification, with ITS2 and rbcL as alternative or complementary regions. These findings will be useful for forensic studies that support the conservation of medicinal plants and their national and global use.",

keywords = "DNA barcoding, medicinal plants, conservation, forensic, Indonesia",

author = "Ria Cahyaningsih and Compton, {Lindsey Jane} and Sri Rahayu and Brehm, {Joana Magos} and Nigel Maxted",

note = "Funding Information: This study was funded by the Ministry of Finance of the Republic of Indonesia, grant number 20160722038259 through the Indonesia Endowment Fund for Education (LPDP) through R. Cahyaningsih{\textquoteright}s scholarship and The APC was funded by the University of Birmingham, UK. Acknowledgments: We thank the Registration and Nursery Subdivision of Bogor Botanic Gardens (BBG) and Cibodas Botanic Gardens (CBD), Indonesian Institute of Sciences (LIPI) and Hortus Botanicus Leiden (HBL), the Netherlands for providing the samples for DNA barcoding. Most of samples are from BBG, except Amomum hochreutineri Valeton, Etlingera solaris (Blume) R.M.Sm., Psychotria montana Blume, Rhododendron macgregoriae F.Muell., Smilax calophylla Wall. ex A.DC. and Staurogyne elongate (Nees) Kuntze are from CBG, and Aglaonema commutatum Schott, Ardisia complanate Wall., Cymbidium ensifolium (L.) Sw., and Hoya diversifolia Blume are from HBL. Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

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N1 - Funding Information: This study was funded by the Ministry of Finance of the Republic of Indonesia, grant number 20160722038259 through the Indonesia Endowment Fund for Education (LPDP) through R. Cahyaningsih’s scholarship and The APC was funded by the University of Birmingham, UK. Acknowledgments: We thank the Registration and Nursery Subdivision of Bogor Botanic Gardens (BBG) and Cibodas Botanic Gardens (CBD), Indonesian Institute of Sciences (LIPI) and Hortus Botanicus Leiden (HBL), the Netherlands for providing the samples for DNA barcoding. Most of samples are from BBG, except Amomum hochreutineri Valeton, Etlingera solaris (Blume) R.M.Sm., Psychotria montana Blume, Rhododendron macgregoriae F.Muell., Smilax calophylla Wall. ex A.DC. and Staurogyne elongate (Nees) Kuntze are from CBG, and Aglaonema commutatum Schott, Ardisia complanate Wall., Cymbidium ensifolium (L.) Sw., and Hoya diversifolia Blume are from HBL. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

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N2 - Over the past decade, plant DNA barcoding has emerged as a scientific breakthrough and is often used to help with species identification or as a taxonomical tool. DNA barcoding is very important in medicinal plant use, not only for identification purposes but also for the authentication of medicinal products. Here, a total of 61 Indonesian medicinal plant species from 30 families and a pair of ITS2, matK, rbcL, and trnL primers were used for a DNA barcoding study consisting of molecular and sequence analyses. This study aimed to analyze how the four identified DNA barcoding regions (ITS2, matK, rbcL, and trnL) aid identification and conservation and to investigate their effectiveness for DNA barcoding for the studied species. This study resulted in 212 DNA barcoding sequences and identified new ones for the studied medicinal plant species. Though there is no ideal or perfect region for DNA barcoding of the target species, we recommend matK as the main region for Indonesian medicinal plant identification, with ITS2 and rbcL as alternative or complementary regions. These findings will be useful for forensic studies that support the conservation of medicinal plants and their national and global use.

AB - Over the past decade, plant DNA barcoding has emerged as a scientific breakthrough and is often used to help with species identification or as a taxonomical tool. DNA barcoding is very important in medicinal plant use, not only for identification purposes but also for the authentication of medicinal products. Here, a total of 61 Indonesian medicinal plant species from 30 families and a pair of ITS2, matK, rbcL, and trnL primers were used for a DNA barcoding study consisting of molecular and sequence analyses. This study aimed to analyze how the four identified DNA barcoding regions (ITS2, matK, rbcL, and trnL) aid identification and conservation and to investigate their effectiveness for DNA barcoding for the studied species. This study resulted in 212 DNA barcoding sequences and identified new ones for the studied medicinal plant species. Though there is no ideal or perfect region for DNA barcoding of the target species, we recommend matK as the main region for Indonesian medicinal plant identification, with ITS2 and rbcL as alternative or complementary regions. These findings will be useful for forensic studies that support the conservation of medicinal plants and their national and global use.

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DNA barcoding medicinal plant species from Indonesia

Abstract

Bibliographical note

Keywords

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