Projects per year
Abstract
Single-domain antibodies, known as nanobodies, have great potential as biorecognition elements for sensors because of their small size, affinity, specificity, and robustness. However, facile and efficient methods of nanobody immobilization are sought that retain their maximum functionality. Herein, we describe the direct immobilization of nanobodies on gold sensors by exploiting a modified cysteine strategically positioned at the C-terminal end of the nanobody. The experimental data based on secondary ion mass spectrometry, circular dichroism, and surface plasmon resonance, taken together with a detailed computational work (molecular dynamics simulations), support the formation of stable and well-oriented nanobody monolayers. Furthermore, the nanobody structure and activity is preserved, wherein the nanobody is immobilized at a high density (approximately 1 nanobody per 13 nm2). The strategy for the spontaneous nanobody self-assembly is simple and effective and possesses exceptional potential to be used in numerous sensing platforms, ranging from clinical diagnosis to environmental monitoring.
Original language | English |
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Pages (from-to) | 17353-17360 |
Number of pages | 8 |
Journal | ACS Applied Materials & Interfaces |
Volume | 13 |
Issue number | 15 |
Early online date | 12 Apr 2021 |
DOIs | |
Publication status | Published - 21 Apr 2021 |
Keywords
- molecular dynamic simulations
- nanobody
- sensor
- single-domain antibody
- surface plasmon resonance
ASJC Scopus subject areas
- Materials Science(all)
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Enabling Next Generation Additive Manufacturing - Lead Nottingham University
Engineering & Physical Science Research Council
1/01/18 → 30/04/24
Project: Research Councils
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FP7_ERC - GLYCOSURF
European Commission, European Commission - Management Costs
1/12/14 → 31/05/21
Project: Research
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Surface-Based Molecular Imprinting for Glycoprotein Recognition
Fossey, J. & Mendes, P.
Engineering & Physical Science Research Council
30/09/13 → 31/05/19
Project: Research Councils