Direct analysis of intact proteins from Escherichia coli colonies by liquid extraction surface analysis mass spectrometry

Elizabeth C. Randall, Josephine Bunch, Helen Cooper

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)
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Abstract

Top-down identification of proteins by liquid extraction surface analysis (LESA) mass spectrometry has previously been reported for tissue sections and dried blood spot samples. Here, we present a modified “contact” LESA method for top-down analysis of proteins directly from living bacterial colonies grown in Petri dishes, without any sample pretreatment. It was possible to identify a number of proteins by use of collision-induced dissociation tandem mass spectrometry followed by searches of the data against an E. coli protein database. The proteins identified suggest that the method may provide insight into the bacterial response to environmental conditions. Moreover, the results show that the “contact” LESA approach results in a smaller sampling area than typical LESA, which may have implications for spatial profiling.
Original languageEnglish
Pages (from-to)10504-10510
JournalAnalytical Chemistry
Volume86
Issue number21
Early online date21 Oct 2014
DOIs
Publication statusPublished - 4 Nov 2014

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