Diol Appended Quenchers for Fluorescein Boronic Acid

Souad Elfeky; SE Flower; N Masumoto; F D'Hooge; L Labarthe; Wenbo Chen; C Len; TD James; John S. Fossey

doi:10.1002/asia.200900386

Diol Appended Quenchers for Fluorescein Boronic Acid

Souad Elfeky, SE Flower, N Masumoto, F D'Hooge, L Labarthe, Wenbo Chen, C Len, TD James, John S. Fossey

Chemistry

Research output: Contribution to journal › Article

27 Citations (Scopus)

Abstract

Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Forster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.

Original language	English
Pages (from-to)	581-588
Number of pages	8
Journal	Chemistry - An Asian Journal
Volume	5
Issue number	3
DOIs	https://doi.org/10.1002/asia.200900386
Publication status	Published - 1 Jan 2010

Keywords

nucleoside
receptor
fluorophore
boronic acid
quencher

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10.1002/asia.200900386

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title = "Diol Appended Quenchers for Fluorescein Boronic Acid",

abstract = "Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Forster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.",

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author = "Souad Elfeky and SE Flower and N Masumoto and F D'Hooge and L Labarthe and Wenbo Chen and C Len and TD James and Fossey, {John S.}",

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TY - JOUR

T1 - Diol Appended Quenchers for Fluorescein Boronic Acid

AU - Elfeky, Souad

AU - Flower, SE

AU - Masumoto, N

AU - D'Hooge, F

AU - Labarthe, L

AU - Chen, Wenbo

AU - Len, C

AU - James, TD

AU - Fossey, John S.

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Forster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.

AB - Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Forster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.

KW - nucleoside

KW - receptor

KW - fluorophore

KW - boronic acid

KW - quencher

U2 - 10.1002/asia.200900386

DO - 10.1002/asia.200900386

M3 - Article

C2 - 20127786

SN - 1861-471X

VL - 5

SP - 581

EP - 588

JO - Chemistry - An Asian Journal

JF - Chemistry - An Asian Journal

IS - 3

ER -

Diol Appended Quenchers for Fluorescein Boronic Acid

Abstract

Keywords

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