Projects per year
Abstract
Nontyphoidal Salmonellae commonly cause fatal bacteraemia in African children lacking anti-Salmonella antibodies. These are facultative intracellular bacteria capable of cell-free and intracellular survival within macrophages. To better understand the relationship between extracellular and intracellular infection in blood and general mechanisms of Ab-related protection against Salmonella, we used human blood and sera to measure kinetics of Ab and complement deposition, serum-mediated bactericidal killing and phagocytosis of invasive African Salmonella enterica serovar Typhimurium D23580. Binding of antibodies peaked by 30 s, but C3 deposition lagged behind, peaking after 2–4 min. C5b-9 deposition was undetectable until between 2 and 6 min and peaked after 10 min, after which time an increase in serum-mediated killing occurred. In contrast, intracellular, opsonized Salmonellae were readily detectable within 5 min. By 10 min, around half of monocytes and most neutrophils contained bacteria. The same kinetics of serum-mediated killing and phagocytosis were observed with S. enterica Typhimurium laboratory strain SL1344, and the S. enterica Enteritidis African invasive isolate D24954 and laboratory strain PT4. The differential kinetics between cell-free killing and phagocytosis of invasive nontyphoidal Salmonella allows these bacteria to escape the blood and establish intracellular infection before they are killed by the membrane attack complex.
Original language | English |
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Pages (from-to) | n/a-n/a |
Journal | European Journal of Immunology |
Volume | 44 |
Issue number | 4 |
Early online date | 20 Jan 2014 |
DOIs | |
Publication status | Published - Apr 2014 |
Keywords
- Antibodies
- Complement
- Opsonization
- Phagocytosis
- Salmonella
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Dive into the research topics of 'Differential timing of antibody-mediated phagocytosis and cell-free killing of invasive African Salmonella allows immune evasion'. Together they form a unique fingerprint.Projects
- 2 Finished
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Selective Biochemical and Synthetic biology approaches for improved delivery of recombinant proteins to the extracellular Milieu
Henderson, I.
Biotechnology & Biological Sciences Research Council
1/12/11 → 31/05/15
Project: Research Councils
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A Bug in the System: How Does Salmonella Induce Novel B Cell Responses?
27/10/08 → 26/10/11
Project: Research Councils