Differential cardiac responses to unilateral sympathetic nerve stimulation in the isolated innervated rabbit heart

J Winter; AS Tanko; KE Brack; John Coote; GA Ng

doi:10.1016/j.autneu.2011.08.004

Differential cardiac responses to unilateral sympathetic nerve stimulation in the isolated innervated rabbit heart

J Winter, AS Tanko, KE Brack, John Coote, GA Ng

Birmingham Medical School

Research output: Contribution to journal › Article

24 Citations (Scopus)

Abstract

The heart receives both a left and right sympathetic innervation. Currently there is no description of an in vitro whole heart preparation for comparing the influence of each sympathetic supply on cardiac function. The aim was to establish the viability of using an in vitro model to investigate the effects of left and right sympathetic chain stimulation (LSS/RSS). For this purpose the upper sympathetic chain on each side was isolated and bipolar stimulating electrodes were attached between T2-T3 and electrically insulated from surrounding tissue in a Langendorff innervated rabbit heart preparation (n = 8). Heart rate (HR) was investigated during sinus rhythm, whilst dromotropic, inotropic and ventricular electrophysiological effects were measured during constant pacing (250 bpm). All responses exhibited linear increases with increases in stimulation frequency (2-10 Hz). The change in HR was larger during RSS than LSS (P

Original language	English
Pages (from-to)	4-14
Number of pages	11
Journal	Autonomic Neuroscience
Volume	166
Issue number	1-2
DOIs	https://doi.org/10.1016/j.autneu.2011.08.004
Publication status	Published - 1 Jan 2012

Keywords

Langendorff heart
Sympathetic innervation
Rabbit
Heterogeneity

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10.1016/j.autneu.2011.08.004

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abstract = "The heart receives both a left and right sympathetic innervation. Currently there is no description of an in vitro whole heart preparation for comparing the influence of each sympathetic supply on cardiac function. The aim was to establish the viability of using an in vitro model to investigate the effects of left and right sympathetic chain stimulation (LSS/RSS). For this purpose the upper sympathetic chain on each side was isolated and bipolar stimulating electrodes were attached between T2-T3 and electrically insulated from surrounding tissue in a Langendorff innervated rabbit heart preparation (n = 8). Heart rate (HR) was investigated during sinus rhythm, whilst dromotropic, inotropic and ventricular electrophysiological effects were measured during constant pacing (250 bpm). All responses exhibited linear increases with increases in stimulation frequency (2-10 Hz). The change in HR was larger during RSS than LSS (P",

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AU - Winter, J

AU - Tanko, AS

AU - Brack, KE

AU - Coote, John

AU - Ng, GA

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AB - The heart receives both a left and right sympathetic innervation. Currently there is no description of an in vitro whole heart preparation for comparing the influence of each sympathetic supply on cardiac function. The aim was to establish the viability of using an in vitro model to investigate the effects of left and right sympathetic chain stimulation (LSS/RSS). For this purpose the upper sympathetic chain on each side was isolated and bipolar stimulating electrodes were attached between T2-T3 and electrically insulated from surrounding tissue in a Langendorff innervated rabbit heart preparation (n = 8). Heart rate (HR) was investigated during sinus rhythm, whilst dromotropic, inotropic and ventricular electrophysiological effects were measured during constant pacing (250 bpm). All responses exhibited linear increases with increases in stimulation frequency (2-10 Hz). The change in HR was larger during RSS than LSS (P

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Differential cardiac responses to unilateral sympathetic nerve stimulation in the isolated innervated rabbit heart

Abstract

Keywords

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