Abstract
Recent studies have shown that, in addition to its role as an adhesion receptor, platelet endothelial cell adhesion molecule 1/CD31 becomes phosphorylated on tyrosine residues Y663 and Y686 and associates with protein tyrosine phosphatases SHP-1 and SHP-2. In this study, we screened for additional proteins which associate with phosphorylated platelet endothelial cell adhesion molecule 1, using surface plasmon resonance. We found that, besides SHP-1 and SHP-2, platelet endothelial cell adhesion molecule 1 binds the cytoplasmic signalling proteins SHIP and PLC-gamma1 via their Src homology 2 domains. Using two phosphopeptides, NSDVQpY663TEVQV and DTETVpY686SEVRK, we demonstrate differential binding of SHP-1, SHP-2, SHIP and PLC-gamma1. All four cytoplasmic signalling proteins directly associate with cellular platelet endothelial cell adhesion molecule 1, immunoprecipitated from pervanadate-stimulated THP-1 cells. These results suggest that overlapping immunoreceptor tyrosine-based inhibition motif/immunoreceptor tyrosine-based activation motif-like motifs within platelet endothelial cell adhesion molecule 1 mediate differential interactions between the Src homology 2 containing signalling proteins SHP-1, SHP-2, SHIP and PLC-gamma1.
Original language | English |
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Pages (from-to) | 77-83 |
Journal | FEBS Letters |
Volume | 450 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 30 Apr 1999 |
Keywords
- Phosphotyrosine
- Intracellular Signaling Peptides and Proteins
- Humans
- Surface Plasmon Resonance
- Phosphoric Monoester Hydrolases
- Amino Acid Sequence
- Protein Tyrosine Phosphatase, Non-Receptor Type 11
- Protein Binding
- Antigens, CD31
- src Homology Domains
- Binding Sites
- Protein Tyrosine Phosphatases
- Phospholipase C gamma
- SH2 Domain-Containing Protein Tyrosine Phosphatases
- Phosphorylation
- Isoenzymes
- Molecular Sequence Data
- Monocytes
- Sequence Homology, Amino Acid
- Phosphopeptides
- Type C Phospholipases
- Vanadates
- Signal Transduction
- Protein Tyrosine Phosphatase, Non-Receptor Type 6