TY - JOUR
T1 - Developmental control of hypoxia during bud burst in grapevine
AU - Meitha, Karlia
AU - Agudelo-Romero, Patricia
AU - Signorelli, Santiago
AU - Gibbs, Daniel J.
AU - Considine, John A.
AU - Foyer, Christine H.
AU - Considine, Michael J.
N1 - This article is protected by copyright. All rights reserved.
PY - 2018/1/15
Y1 - 2018/1/15
N2 - Dormant or quiescent buds of woody perennials are often dense, and in the case of grapevine (Vitis vinifera L.) have a low tissue oxygen status. The precise timing of the decision to resume growth is difficult to predict, but once committed the increase in tissue oxygen status is rapid and developmentally regulated. Here we show that more than a third of the grapevine homologues of widely conserved hypoxia-responsive genes, and nearly a fifth of all grapevine genes possessing a plant hypoxia-responsive promoter element were differentially regulated during bud burst, in apparent harmony with resumption of meristem identity and cell-cycle gene regulation. We then investigated the molecular and biochemical properties of the grapevine ERF-VII homologues, which in other species are oxygen labile and function in transcriptional regulation of hypoxia-responsive genes. Each of the three VvERF-VIIs were substrates for oxygen-dependent proteolysis in vitro, as a function of the N-terminal cysteine. Collectively these data support an important developmental function of oxygen-dependent signalling in determining the timing and effective coordination bud burst in grapevine. In addition, novel regulators, including GASA-, TCP-, MYB3R-, PLT- and WUS-like transcription factors, were identified as hallmarks of the orderly and functional resumption of growth following quiescence in buds.
AB - Dormant or quiescent buds of woody perennials are often dense, and in the case of grapevine (Vitis vinifera L.) have a low tissue oxygen status. The precise timing of the decision to resume growth is difficult to predict, but once committed the increase in tissue oxygen status is rapid and developmentally regulated. Here we show that more than a third of the grapevine homologues of widely conserved hypoxia-responsive genes, and nearly a fifth of all grapevine genes possessing a plant hypoxia-responsive promoter element were differentially regulated during bud burst, in apparent harmony with resumption of meristem identity and cell-cycle gene regulation. We then investigated the molecular and biochemical properties of the grapevine ERF-VII homologues, which in other species are oxygen labile and function in transcriptional regulation of hypoxia-responsive genes. Each of the three VvERF-VIIs were substrates for oxygen-dependent proteolysis in vitro, as a function of the N-terminal cysteine. Collectively these data support an important developmental function of oxygen-dependent signalling in determining the timing and effective coordination bud burst in grapevine. In addition, novel regulators, including GASA-, TCP-, MYB3R-, PLT- and WUS-like transcription factors, were identified as hallmarks of the orderly and functional resumption of growth following quiescence in buds.
KW - Journal Article
KW - bud burst
KW - hypoxia
KW - redox
KW - quiescence
KW - woody perennial plant
KW - transcriptome
KW - meristem
KW - cell cycle
KW - ethylene response factor
KW - N-end rule proteolysis
U2 - 10.1111/pce.13141
DO - 10.1111/pce.13141
M3 - Article
C2 - 29336037
SN - 0140-7791
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
ER -