Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

T W Bilverstone; N L Kinsmore; N P Minton; Sarah Kuehne

doi:10.1016/j.anaerobe.2017.01.009

Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

T W Bilverstone
, N L Kinsmore
, N P Minton
, Sarah Kuehne

Dentistry

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

188 Downloads (Pure)

Abstract

Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

Original language	English
Pages (from-to)	51-54
Number of pages	4
Journal	Anaerobe
Volume	44
Early online date	17 Jan 2017
DOIs	https://doi.org/10.1016/j.anaerobe.2017.01.009
Publication status	Published - Apr 2017

Keywords

ADP Ribose Transferases
Animals
Bacterial Proteins
Cell Survival
Cercopithecus aethiops
Clostridium difficile
Gene Deletion
Gene Expression Regulation, Bacterial
Genes, Regulator
Vero Cells
Journal Article

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title = "Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels",

abstract = "Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.",

keywords = "ADP Ribose Transferases, Animals, Bacterial Proteins, Cell Survival, Cercopithecus aethiops, Clostridium difficile, Gene Deletion, Gene Expression Regulation, Bacterial, Genes, Regulator, Vero Cells, Journal Article",

author = "Bilverstone, \{T W\} and Kinsmore, \{N L\} and Minton, \{N P\} and Sarah Kuehne",

year = "2017",

month = apr,

doi = "10.1016/j.anaerobe.2017.01.009",

language = "English",

volume = "44",

pages = "51--54",

journal = "Anaerobe",

issn = "1075-9964",

publisher = "Elsevier",

}

TY - JOUR

T1 - Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

AU - Bilverstone, T W

AU - Kinsmore, N L

AU - Minton, N P

AU - Kuehne, Sarah

PY - 2017/4

Y1 - 2017/4

N2 - Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

AB - Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

KW - ADP Ribose Transferases

KW - Animals

KW - Bacterial Proteins

KW - Cell Survival

KW - Cercopithecus aethiops

KW - Clostridium difficile

KW - Gene Deletion

KW - Gene Expression Regulation, Bacterial

KW - Genes, Regulator

KW - Vero Cells

KW - Journal Article

UR - https://www.scopus.com/pages/publications/85012256194

U2 - 10.1016/j.anaerobe.2017.01.009

DO - 10.1016/j.anaerobe.2017.01.009

M3 - Article

C2 - 28108389

SN - 1075-9964

VL - 44

SP - 51

EP - 54

JO - Anaerobe

JF - Anaerobe

ER -

Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

Abstract

Keywords

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