Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

T W Bilverstone; N L Kinsmore; N P Minton; Sarah Kuehne

doi:10.1016/j.anaerobe.2017.01.009

Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

T W Bilverstone, N L Kinsmore, N P Minton, Sarah Kuehne

Dentistry

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

149 Downloads (Pure)

Abstract

Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

Original language	English
Pages (from-to)	51-54
Number of pages	4
Journal	Anaerobe
Volume	44
Early online date	17 Jan 2017
DOIs	https://doi.org/10.1016/j.anaerobe.2017.01.009
Publication status	Published - Apr 2017

Keywords

ADP Ribose Transferases
Animals
Bacterial Proteins
Cell Survival
Cercopithecus aethiops
Clostridium difficile
Gene Deletion
Gene Expression Regulation, Bacterial
Genes, Regulator
Vero Cells
Journal Article

Access to Document

10.1016/j.anaerobe.2017.01.009Licence: Creative Commons: Attribution (CC BY)

Bilverstone_et_al_Development_Clostridium_AnaerobeFinal published version, 612 KBLicence: Creative Commons: Attribution (CC BY)

Cite this

@article{4e47ec35f2ae4b20a7f7a3bdb1226b47,

title = "Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels",

abstract = "Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.",

keywords = "ADP Ribose Transferases, Animals, Bacterial Proteins, Cell Survival, Cercopithecus aethiops, Clostridium difficile, Gene Deletion, Gene Expression Regulation, Bacterial, Genes, Regulator, Vero Cells, Journal Article",

author = "Bilverstone, {T W} and Kinsmore, {N L} and Minton, {N P} and Sarah Kuehne",

year = "2017",

month = apr,

doi = "10.1016/j.anaerobe.2017.01.009",

language = "English",

volume = "44",

pages = "51--54",

journal = "Anaerobe",

issn = "1075-9964",

publisher = "Elsevier",

}

TY - JOUR

T1 - Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

AU - Bilverstone, T W

AU - Kinsmore, N L

AU - Minton, N P

AU - Kuehne, Sarah

PY - 2017/4

Y1 - 2017/4

N2 - Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

AB - Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291ΔPaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

KW - ADP Ribose Transferases

KW - Animals

KW - Bacterial Proteins

KW - Cell Survival

KW - Cercopithecus aethiops

KW - Clostridium difficile

KW - Gene Deletion

KW - Gene Expression Regulation, Bacterial

KW - Genes, Regulator

KW - Vero Cells

KW - Journal Article

U2 - 10.1016/j.anaerobe.2017.01.009

DO - 10.1016/j.anaerobe.2017.01.009

M3 - Article

C2 - 28108389

SN - 1075-9964

VL - 44

SP - 51

EP - 54

JO - Anaerobe

JF - Anaerobe

ER -

Development of Clostridium difficile R20291ΔPaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

Abstract

Keywords

Access to Document

Fingerprint

Cite this