Detection of single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer using a fluorophore-tagged DNA probe

ZY Zhao; M San; JL Duprey; John Arrand; JS Vyle; James Tucker

doi:10.1016/j.bmcl.2011.11.053

Detection of single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer using a fluorophore-tagged DNA probe

ZY Zhao, M San, JL Duprey, John Arrand, JS Vyle, James Tucker

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer were identified using oligodeoxynucleotide probe sequences bearing internal anthracene fluorophores proximal to the SNP site. Depending upon the nature of the synthesised target sequences, probe-target duplex formation could lead to enhanced or attenuated fluorescence emission from the anthracene, enabling detection of a proximal base-pair as either matching or mismatching.

Original language	English
Pages (from-to)	129-132
Number of pages	4
Journal	Bioorganic & Medicinal Chemistry Letters
Volume	22
Issue number	1
DOIs	https://doi.org/10.1016/j.bmcl.2011.11.053
Publication status	Published - 1 Jan 2012

Keywords

DNA
SNP
Fluorescence
Sensing
Nucleic acids

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bmcl.2011.11.053Licence: None: All rights reserved

Functional DNA-based assemblies
Tucker, J.
Engineering & Physical Science Research Council
16/03/09 → 31/03/14
Project: Research Councils

Cite this

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title = "Detection of single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer using a fluorophore-tagged DNA probe",

abstract = "Single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer were identified using oligodeoxynucleotide probe sequences bearing internal anthracene fluorophores proximal to the SNP site. Depending upon the nature of the synthesised target sequences, probe-target duplex formation could lead to enhanced or attenuated fluorescence emission from the anthracene, enabling detection of a proximal base-pair as either matching or mismatching.",

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AU - Zhao, ZY

AU - San, M

AU - Duprey, JL

AU - Arrand, John

AU - Vyle, JS

AU - Tucker, James

PY - 2012/1/1

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N2 - Single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer were identified using oligodeoxynucleotide probe sequences bearing internal anthracene fluorophores proximal to the SNP site. Depending upon the nature of the synthesised target sequences, probe-target duplex formation could lead to enhanced or attenuated fluorescence emission from the anthracene, enabling detection of a proximal base-pair as either matching or mismatching.

AB - Single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer were identified using oligodeoxynucleotide probe sequences bearing internal anthracene fluorophores proximal to the SNP site. Depending upon the nature of the synthesised target sequences, probe-target duplex formation could lead to enhanced or attenuated fluorescence emission from the anthracene, enabling detection of a proximal base-pair as either matching or mismatching.

KW - DNA

KW - SNP

KW - Fluorescence

KW - Sensing

KW - Nucleic acids

U2 - 10.1016/j.bmcl.2011.11.053

DO - 10.1016/j.bmcl.2011.11.053

M3 - Article

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JO - Bioorganic & Medicinal Chemistry Letters

JF - Bioorganic & Medicinal Chemistry Letters

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ER -

Detection of single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer using a fluorophore-tagged DNA probe

Abstract

Keywords

UN SDGs

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Projects

Functional DNA-based assemblies

Cite this