Detection by whole genome microarrays of a spontaneous 126-gene deletion during construction of a ytfE mutant: confirmation that a ytfE mutation results in loss of repair of iron-sulfur centres in proteins damaged by oxidative or nitrosative stress.

Claire Vine; MC Justino; LM Saraiva; Jeffrey Cole

doi:10.1016/j.mimet.2010.01.023

Detection by whole genome microarrays of a spontaneous 126-gene deletion during construction of a ytfE mutant: confirmation that a ytfE mutation results in loss of repair of iron-sulfur centres in proteins damaged by oxidative or nitrosative stress.

Claire Vine
, MC Justino
, LM Saraiva
, Jeffrey Cole

Biosciences

Research output: Contribution to journal › Article

22 Citations (Scopus)

Abstract

We show that genomic hybridization allows detection of a spontaneous secondary deletion of 126 genes that occurred during construction of an Escherichia coli ytfE mutant, LMS4209, explaining some of its unexpected growth defects. We confirm that YtfE is required to repair damage to iron-sulfur centres and for hydrogen peroxide resistance.

Original language	English
Pages (from-to)	77-9
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	81
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2010.01.023
Publication status	Published - 1 Apr 2010

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Vine, C., Justino, MC., Saraiva, LM., & Cole, J. (2010). Detection by whole genome microarrays of a spontaneous 126-gene deletion during construction of a ytfE mutant: confirmation that a ytfE mutation results in loss of repair of iron-sulfur centres in proteins damaged by oxidative or nitrosative stress. Journal of Microbiological Methods, 81(1), 77-9. https://doi.org/10.1016/j.mimet.2010.01.023

Vine, Claire ; Justino, MC ; Saraiva, LM et al. / Detection by whole genome microarrays of a spontaneous 126-gene deletion during construction of a ytfE mutant: confirmation that a ytfE mutation results in loss of repair of iron-sulfur centres in proteins damaged by oxidative or nitrosative stress. In: Journal of Microbiological Methods. 2010 ; Vol. 81, No. 1. pp. 77-9.

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abstract = "We show that genomic hybridization allows detection of a spontaneous secondary deletion of 126 genes that occurred during construction of an Escherichia coli ytfE mutant, LMS4209, explaining some of its unexpected growth defects. We confirm that YtfE is required to repair damage to iron-sulfur centres and for hydrogen peroxide resistance.",

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Vine, C, Justino, MC, Saraiva, LM & Cole, J 2010, 'Detection by whole genome microarrays of a spontaneous 126-gene deletion during construction of a ytfE mutant: confirmation that a ytfE mutation results in loss of repair of iron-sulfur centres in proteins damaged by oxidative or nitrosative stress.', Journal of Microbiological Methods, vol. 81, no. 1, pp. 77-9. https://doi.org/10.1016/j.mimet.2010.01.023

Detection by whole genome microarrays of a spontaneous 126-gene deletion during construction of a ytfE mutant: confirmation that a ytfE mutation results in loss of repair of iron-sulfur centres in proteins damaged by oxidative or nitrosative stress. / Vine, Claire; Justino, MC; Saraiva, LM et al.
In: Journal of Microbiological Methods, Vol. 81, No. 1, 01.04.2010, p. 77-9.

Research output: Contribution to journal › Article

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AB - We show that genomic hybridization allows detection of a spontaneous secondary deletion of 126 genes that occurred during construction of an Escherichia coli ytfE mutant, LMS4209, explaining some of its unexpected growth defects. We confirm that YtfE is required to repair damage to iron-sulfur centres and for hydrogen peroxide resistance.

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DO - 10.1016/j.mimet.2010.01.023

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Vine C, Justino MC, Saraiva LM, Cole J. Detection by whole genome microarrays of a spontaneous 126-gene deletion during construction of a ytfE mutant: confirmation that a ytfE mutation results in loss of repair of iron-sulfur centres in proteins damaged by oxidative or nitrosative stress. Journal of Microbiological Methods. 2010 Apr 1;81(1):77-9. doi: 10.1016/j.mimet.2010.01.023