Detecting transient trapping from a single trajectory: a structural approach

Yann Lanoiselee, Jak Grimes, Zsombor Koszegi, Davide Calebiro

Research output: Contribution to journalArticlepeer-review

71 Downloads (Pure)


In this article, we introduce a new method to detect transient trapping events within a single particle trajectory, thus allowing the explicit accounting of changes in the particle’s dynamics over time. Our method is based on new measures of a smoothed recurrence matrix. The newly introduced set of measures takes into account both the spatial and temporal structure of the trajectory. Therefore, it is adapted to study short-lived trapping domains that are not visited by multiple trajectories. Contrary to most existing methods, it does not rely on using a window, sliding along the trajectory, but rather investigates the trajectory as a whole. This method provides useful information to study intracellular and plasma membrane compartmentalisation. Additionally, this method is applied to single particle trajectory data of β2-adrenergic receptors, revealing that receptor stimulation results in increased trapping of receptors in defined domains, without changing the diffusion of free receptors.
Original languageEnglish
Article number1044
Number of pages16
Issue number8
Publication statusPublished - 13 Aug 2021

Bibliographical note

Funding Information:
Funding: This work was partially supported by a Wellcome Trust Senior Research Fellowship (212313/Z/18/Z to D.C.).

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.


  • single particle trajectory
  • stochastic processes
  • trapping
  • confinement


Dive into the research topics of 'Detecting transient trapping from a single trajectory: a structural approach'. Together they form a unique fingerprint.

Cite this