TY - JOUR
T1 - Design, synthesis and characterisation of a Peptide with Oxaloacetate Decarboxylase activity
AU - Taylor, Susan
AU - Rutherford, Trevor
AU - Allemann, Rudolf
PY - 2001/10/8
Y1 - 2001/10/8
N2 - The synthesis of Oxaldie-3. a synthetic 31-residue peptide with oxaloacetate decarboxylase activity, is described. Biophysical characterisation by gel filtration, CD and NMR spectroscopy indicated that the peptide adopted a folded structure in solution. Oxaldie-3 was an efficient catalyst at concentrations as low as 2 muM, 100-fold lower than the previously described Oxaldie-2, which relied on aggregating alpha -helices for activity. Oxaldie-3 speeded decarboxylation by more than three orders of magnitude relative to simple amines. (C) 2001 Elsevier Science Ltd. All rights reserved.
AB - The synthesis of Oxaldie-3. a synthetic 31-residue peptide with oxaloacetate decarboxylase activity, is described. Biophysical characterisation by gel filtration, CD and NMR spectroscopy indicated that the peptide adopted a folded structure in solution. Oxaldie-3 was an efficient catalyst at concentrations as low as 2 muM, 100-fold lower than the previously described Oxaldie-2, which relied on aggregating alpha -helices for activity. Oxaldie-3 speeded decarboxylation by more than three orders of magnitude relative to simple amines. (C) 2001 Elsevier Science Ltd. All rights reserved.
UR - http://www.scopus.com/inward/record.url?scp=0035829134&partnerID=8YFLogxK
U2 - 10.1016/S0960-894X(01)00519-4
DO - 10.1016/S0960-894X(01)00519-4
M3 - Article
VL - 11
SP - 2631
EP - 2635
JO - Bioorganic & Medicinal Chemistry Letters
JF - Bioorganic & Medicinal Chemistry Letters
IS - 19
ER -