Dendritic cell-based proliferative assays of peripheral T cell responses to tetanus toxoid

Parth Narendran; Kathryn Elsegood; Nicola J Leech; Colin M Dayan

Dendritic cell-based proliferative assays of peripheral T cell responses to tetanus toxoid

Parth Narendran, Kathryn Elsegood, Nicola J Leech, Colin M Dayan

Metabolism and Systems Science

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Antigen-specific proliferative responses of peripheral blood T cells are widely used in humans to study the T cell compartment. However, responses to autoantigens are often weak and poorly reproducible. Here we show, using a test recall antigen (tetanus toxoid), that absolute levels of proliferation, minimally detectable antigen doses, and/or ability to detect statistically significant responses can be enhanced using in vitro-generated autologous dendritic cells as antigen presenting cells.

Original language	English
Pages (from-to)	170-4
Number of pages	5
Journal	Annals of the New York Academy of Sciences
Volume	958
Publication status	Published - Apr 2002

Keywords

Antigen Presentation
Autoantigens
Cell Division
Cells, Cultured
Dendritic Cells
Diabetes Mellitus, Type 1
Flow Cytometry
Humans
T-Lymphocytes
Tetanus Toxoid
Vaccination

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Dendritic cell-based proliferative assays of peripheral T cell responses to tetanus toxoid",

abstract = "Antigen-specific proliferative responses of peripheral blood T cells are widely used in humans to study the T cell compartment. However, responses to autoantigens are often weak and poorly reproducible. Here we show, using a test recall antigen (tetanus toxoid), that absolute levels of proliferation, minimally detectable antigen doses, and/or ability to detect statistically significant responses can be enhanced using in vitro-generated autologous dendritic cells as antigen presenting cells.",

keywords = "Antigen Presentation, Autoantigens, Cell Division, Cells, Cultured, Dendritic Cells, Diabetes Mellitus, Type 1, Flow Cytometry, Humans, T-Lymphocytes, Tetanus Toxoid, Vaccination",

author = "Parth Narendran and Kathryn Elsegood and Leech, {Nicola J} and Dayan, {Colin M}",

year = "2002",

month = apr,

language = "English",

volume = "958",

pages = "170--4",

journal = "Annals of the New York Academy of Sciences",

issn = "0077-8923",

publisher = "Wiley",

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TY - JOUR

T1 - Dendritic cell-based proliferative assays of peripheral T cell responses to tetanus toxoid

AU - Narendran, Parth

AU - Elsegood, Kathryn

AU - Leech, Nicola J

AU - Dayan, Colin M

PY - 2002/4

Y1 - 2002/4

N2 - Antigen-specific proliferative responses of peripheral blood T cells are widely used in humans to study the T cell compartment. However, responses to autoantigens are often weak and poorly reproducible. Here we show, using a test recall antigen (tetanus toxoid), that absolute levels of proliferation, minimally detectable antigen doses, and/or ability to detect statistically significant responses can be enhanced using in vitro-generated autologous dendritic cells as antigen presenting cells.

AB - Antigen-specific proliferative responses of peripheral blood T cells are widely used in humans to study the T cell compartment. However, responses to autoantigens are often weak and poorly reproducible. Here we show, using a test recall antigen (tetanus toxoid), that absolute levels of proliferation, minimally detectable antigen doses, and/or ability to detect statistically significant responses can be enhanced using in vitro-generated autologous dendritic cells as antigen presenting cells.

KW - Antigen Presentation

KW - Autoantigens

KW - Cell Division

KW - Cells, Cultured

KW - Dendritic Cells

KW - Diabetes Mellitus, Type 1

KW - Flow Cytometry

KW - Humans

KW - T-Lymphocytes

KW - Tetanus Toxoid

KW - Vaccination

M3 - Article

C2 - 12021100

SN - 0077-8923

VL - 958

SP - 170

EP - 174

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

ER -

Dendritic cell-based proliferative assays of peripheral T cell responses to tetanus toxoid

Abstract

Keywords

UN SDGs

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