Decrease of serum protein concentration difference by solid-phase ligand libraries

Yi Jun Qi, Rao Hua Niu, Pan Wang, Yan Fang Hou, Wei Xia Chao, Yuan Fang Ma, D. G. Ward, P. J. Johnson

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To determine the effects of solid-phase ligand libraries on enrichment of low-abundance serum protein and improve the low-abundance serum protein identification by mass spectrometer(MS). Methods: Three pooled serum samples were treated with ProteoMiner and then subjected to one-dimensional (one-D) or two-dimensional (two-D) SDS-PAGE followed by comparative image analysis. The remarkable increased protein spots on two-D map were identified by high performance liquid chromatogrphy ( HPLC ) coupled on-line to ESI/MS-MS. Results: The one-D and two-D gel map revealed more low-abundance protein bands and protein spots, respectively. MS identification of significantly enriched protein spots on two-D gel improved low-abundance protein identification after library beads treatment, e. g. clusterin, vitronectin, C4b-binding protein alpha chain, complement C4-A. Conclusion: The technique of solid-phase ligand libraries can enrich serum low-abundance proteins whilst decrease high-abundance protein concentration, which indicates that this method is useful for enrichment of low-abundance protein and identification.

Original languageEnglish
Pages (from-to)770-774
Number of pages5
JournalActa Anatomica Sinica
Volume42
Issue number6
DOIs
Publication statusPublished - 6 Dec 2011

Keywords

  • Clusterin
  • High performance liquid chromatography
  • High-abundance protein
  • Low-abundance protein
  • Serum proteomics
  • Solid-phase ligand library

ASJC Scopus subject areas

  • Anatomy
  • Structural Biology
  • Histology
  • Embryology

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