Cuticular conductance of adaxial and abaxial leaf surfaces and its relation to minimum leaf surface conductance

Diego Marquez, Hilary Stuart-Williams, Graham D. Farquhar, Florian Busch

Research output: Contribution to journalArticlepeer-review

35 Downloads (Pure)

Abstract

Cuticular conductance to water (g cw) is difficult to quantify for stomatous surfaces due to the complexity of separating cuticular and stomatal transpiration, and additional complications arise for determining adaxial and abaxial g cw. This has led to the neglect of g cw as a separate parameter in most common gas exchange measurements. Here, we describe a simple technique to simultaneously estimate adaxial and abaxial values of g cw, tested in two amphistomatous plant species. What we term the ‘Red-Light method’ is used to estimate g cw from gas exchange measurements and a known CO 2 concentration inside the leaf during photosynthetic induction under red light. We provide an easy-to-use web application to assist with the calculation of g cw. While adaxial and abaxial g cw varies significantly between leaves of the same species we found that the ratio of adaxial/abaxial g cwn) is stable within a plant species. This has implications for use of generic values of g cw when analysing gas exchange data. The Red-Light method can be used to estimate total cuticular conductance (g cw-T) accurately with the most common setup of gas exchange instruments, i.e. a chamber mixing the adaxial and abaxial gases, allowing for a wide application of this technique.

Original languageEnglish
JournalNew Phytologist
Early online date30 Jun 2021
DOIs
Publication statusE-pub ahead of print - 30 Jun 2021

Keywords

  • abaxial cuticular conductance
  • adaxial cuticular conductance
  • amphistomatous leaf
  • cuticular conductance to water
  • leaf gas exchange
  • nocturnal conductance
  • photosynthetic induction

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Fingerprint

Dive into the research topics of 'Cuticular conductance of adaxial and abaxial leaf surfaces and its relation to minimum leaf surface conductance'. Together they form a unique fingerprint.

Cite this