Cryptic transmission of ST405 Escherichia coli carrying blaNDM-4 in hospital

Xiaoxia Zhang; Yu Feng; Weilong Zhou; Alan McNally; Zhiyong Zong

doi:10.1038/s41598-017-18910-w

Cryptic transmission of ST405 Escherichia coli carrying bla_NDM-4 in hospital

Xiaoxia Zhang
, Yu Feng
, Weilong Zhou
, Alan McNally
, Zhiyong Zong^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

234 Downloads (Pure)

Abstract

Three carbapenem-resistant Escherichia coli were recovered from rectal swabs of different patients in a tertiary hospital and were found carrying bla_NDM-4, an uncommon bla_NDM variant. Genome sequences of the isolates were obtained using Illumina technology and the long-read MinION sequencer. The isolates belonged to ST405 and phylogenetic group D, a globally distributed lineage associated with antimicrobial resistance. In addition to bla_NDM-4, the three isolates carried 14 known resistance genes including the extended-spectrum β-lactamase gene bla_CTX-M-15. There were only 1 or 2 SNPs between the isolates, suggesting a common origin and cryptic transmission in hospital. bla_NDM-4 was located on a 46.5-kb IncFIA self-transmissible plasmid, which may facilitate further dissemination of bla_NDM-4. Two copies of IS26 bracketed a 14.6-kb region containing bla_NDM-4 and have the potential to form a composite transposon for mediating the mobilization of bla_NDM-4.

Original language	English
Article number	390
Journal	Scientific Reports
Volume	8
DOIs	https://doi.org/10.1038/s41598-017-18910-w
Publication status	Published - 10 Jan 2018

Keywords

Antimicrobial resistance
Bacterial Infection

ASJC Scopus subject areas

General

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Cite this

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title = "Cryptic transmission of ST405 Escherichia coli carrying blaNDM-4 in hospital",

abstract = "Three carbapenem-resistant Escherichia coli were recovered from rectal swabs of different patients in a tertiary hospital and were found carrying blaNDM-4, an uncommon blaNDM variant. Genome sequences of the isolates were obtained using Illumina technology and the long-read MinION sequencer. The isolates belonged to ST405 and phylogenetic group D, a globally distributed lineage associated with antimicrobial resistance. In addition to blaNDM-4, the three isolates carried 14 known resistance genes including the extended-spectrum β-lactamase gene blaCTX-M-15. There were only 1 or 2 SNPs between the isolates, suggesting a common origin and cryptic transmission in hospital. blaNDM-4 was located on a 46.5-kb IncFIA self-transmissible plasmid, which may facilitate further dissemination of blaNDM-4. Two copies of IS26 bracketed a 14.6-kb region containing blaNDM-4 and have the potential to form a composite transposon for mediating the mobilization of blaNDM-4.",

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author = "Xiaoxia Zhang and Yu Feng and Weilong Zhou and Alan McNally and Zhiyong Zong",

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AU - Zhang, Xiaoxia

AU - Feng, Yu

AU - Zhou, Weilong

AU - McNally, Alan

AU - Zong, Zhiyong

PY - 2018/1/10

Y1 - 2018/1/10

N2 - Three carbapenem-resistant Escherichia coli were recovered from rectal swabs of different patients in a tertiary hospital and were found carrying blaNDM-4, an uncommon blaNDM variant. Genome sequences of the isolates were obtained using Illumina technology and the long-read MinION sequencer. The isolates belonged to ST405 and phylogenetic group D, a globally distributed lineage associated with antimicrobial resistance. In addition to blaNDM-4, the three isolates carried 14 known resistance genes including the extended-spectrum β-lactamase gene blaCTX-M-15. There were only 1 or 2 SNPs between the isolates, suggesting a common origin and cryptic transmission in hospital. blaNDM-4 was located on a 46.5-kb IncFIA self-transmissible plasmid, which may facilitate further dissemination of blaNDM-4. Two copies of IS26 bracketed a 14.6-kb region containing blaNDM-4 and have the potential to form a composite transposon for mediating the mobilization of blaNDM-4.

AB - Three carbapenem-resistant Escherichia coli were recovered from rectal swabs of different patients in a tertiary hospital and were found carrying blaNDM-4, an uncommon blaNDM variant. Genome sequences of the isolates were obtained using Illumina technology and the long-read MinION sequencer. The isolates belonged to ST405 and phylogenetic group D, a globally distributed lineage associated with antimicrobial resistance. In addition to blaNDM-4, the three isolates carried 14 known resistance genes including the extended-spectrum β-lactamase gene blaCTX-M-15. There were only 1 or 2 SNPs between the isolates, suggesting a common origin and cryptic transmission in hospital. blaNDM-4 was located on a 46.5-kb IncFIA self-transmissible plasmid, which may facilitate further dissemination of blaNDM-4. Two copies of IS26 bracketed a 14.6-kb region containing blaNDM-4 and have the potential to form a composite transposon for mediating the mobilization of blaNDM-4.

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